Preparation optimization for two inclusion compounds of erinacine A and their stabilities
10.3969/j.issn.1001-1528.2018.01.015
- VernacularTitle:猴头菌素A2种包合物制备工艺的优化及其稳定性
- Author:
Ruo-Xi LIU
1
;
Ping WANG
;
Jin-Zhe HE
Author Information
1. 浙江工业大学药学院
- Keywords:
erinacine A;
inclusion compounds;
β-cyclodextrin;
hydroxypropyl-β-cyclodextrin;
preparation;
orthogonal test;
stabilities
- From:
Chinese Traditional Patent Medicine
2018;40(1):80-86
- CountryChina
- Language:Chinese
-
Abstract:
AIM To optimize the preparation of two inclusion compounds of erinacine A and to evaluate their stabilities.METHODS With feed ratio,milling time and milling speed as influencing factors,inclusion rate and yield as evaluation indices,the preparation was optimized by orthogonal test.The obtained inclusion compounds were characterized by infrared spectrophotometry and TLC,whose stabilities at high temperature (60 ℃),strong light (3 000 1x) and high humidity [(90 ±5)％] were investigated.RESULTS The optimal β-cyclodextrin inclusion conditions were determined to be 5 ∶ 1 for feed ratio,60 min for milling time,and 300 r/min for milling speed,the inclusion rate and yield were 20.66％ and 88.21％,respectively.The optimal hydroxypropyl-β-cyclodextrin inclusion conditions were determined to be 25 ∶ 1 for feed ratio,90 min for milling time,and 400 r/min for milling speed,the inclusion rate and yield were 69.25％ and 96.31％,respectively.The inclusion of Hericium erinaceus 80％ ethanol extract was physical process without composition change.At high temperature and strong light,two inclusion compounds' appearance showed no obvious change with little loss of erinacine A,which was just the contrary at high humidity.The inclusion effect of hydroxypropyl-β-cyclodextrin inclusion compound was better than that of β-cyclodextrin inclusion compound.CONCLUSION Both β-cyclodextrin and hydroxypropyl-β-cyclodextrin inclusion compounds of erinacine A exhibit good heat stability and light stability,but deliquescence can easily happen to them.
