Simultaneous determination of four constituents in Kesuting Capsules by HPLC
10.3969/j.issn.1001-1528.2017.01.020
- VernacularTitle:HPLC法同时测定咳速停胶囊中4种成分
- Author:
Yi LUO
1
;
Wen-Li YAO
;
Lin-Lin WU
;
Juan-Yan YANG
;
Xiang-Jun MAO
Author Information
1. 贵州医科大学
- Keywords:
Kesuting Capsules;
morphine;
ephedrine hydrochloride;
pseudoephedrine hydrochloride;
codeine phosphate;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(1):102-106
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Kesuting Capsules (a fast cough suppressant,containing Ephedrae Herba,Papaveris Pericarpium,Platycodonis Radix,etc.).METHODS The analysis of trichloromethane-strong ammonia extract of Kesuting Capsules was performed on a 35 ℃ thermostatic Welch Ultimate(◎) XB-C18 column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.01 mol/L potassium dihydrogen phosphate buffer flowing at 1.0 mL/min in an isocratic elution manner,and the detection wavelength was set at 210 nm.RESULTS Morphine,ephedrine hydrochloride,pseudoephedrine hydrochloride and codeine phosphate showed good linear relationships within the ranges of 8.054-67.12 μg/mL (r =0.999 5),22.31-185.9 μg/mL (r =0.999 9),21.26-177.2 μg/mL (r =0.999 7) and 1.212-10.09 μg/mL (r =0.999 7),whose average recoveries (n =9) were 100.9％ (RSD =2.0％),101.4％ (RSD =3.6％),105.3％ (RSD =1.2％) and 106.2％ (RSD =1.2％),respectively.CONCLUSION This simple method can be used for the rapid quality control of Kesuting Capsules.
