Simultaneous determination of four constituents in Yangxin Dingji Capsules by HPLC
10.3969/j.issn.1001-1528.2017.01.019
- VernacularTitle:HPLC法同时测定养心定悸胶囊中4种成分
- Author:
Han-Xue WANG
1
;
Lin-Feng LI
;
Rui AN
;
Xue-Mei CHENG
;
Chang-Hong WANG
;
Xin-Hong WANG
Author Information
1. 上海中医药大学中药学院
- Keywords:
Yangxin Dingji Capsules;
liquiritin;
glycyrrhizic acid;
cinnamic acid;
cinnamic aldehyde;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(1):98-101
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Yangxin Dingji Capsules (a cardiac tonic for palpitation,containing Glycyrrhizae Radix et Rhizoma Praeparata cum Melle,Cinnamomi Ramulus,Rehmanniae Radix,etc.).METHODS The analysis of 50％ methanol extract of Yangxin Dingji Capsules was performed on a 40 ℃ thermostatic Diamonsil C1s column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of 0.1％ formic acid-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 265 nm.RESULTS Liquiritin,glycyrrhizic acid,cinnamic acid and cinnamic aldehyde showed good linear relationships within the ranges of 1.00-80.24 μg/mL (r=0.999 0),2.52-100.70 μg/mL (r--0.999 7),0.50-40.40 μg/mL (r =1.000 0) and 0.66-52.96 μg/mL (r =1.000 0),whose average recoveries were 97.74％,100.97％,101.48％ and 99.49％ with the RSDs of 0.45％,1.11％,1.27％ and 1.66％,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Yangxin Dingji Capsules.
