Simultaneous determination of four constituents in Kaihoujian Spray by GC-FID
10.3969/j.issn.1001-1528.2017.01.018
- VernacularTitle:GC-FID法同时测定开喉剑喷雾剂中4种成分
- Author:
Gui-Qin DU
1
;
Ya-Min ZUO
;
Wei PAN
;
Xing LI
;
Hong-Hong TIAN
;
Qing WU
;
Ying DU
Author Information
1. 贵州医科大学医学检验学院
- Keywords:
Kaihoujian Spray;
menthol;
matrine;
sophocarpine;
sophoridine;
GC-FID
- From:
Chinese Traditional Patent Medicine
2017;39(1):94-97
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a gas chromatography-flame ionization detector (GC-FID) method for the simultaneous content determination of four constituents in Kaihoujian Spray (a spray for management of oral and laryngopharyngeal inflammatory condition,containing Sophorae tonkinensis Radix et Rhizoma,Cicadae Periostracum,menthol,etc.).METHODS The analysis of mixure of Kaihoujian Spray and internal standard (dibutyl phthalate) solution was conducted on an HP-INNOWax column (30 m ×0.25 mm ×0.25 μm),both of the injector temperature and detector temperature were set at 260 ℃,the split ratio was 5 ∶ 1,and the flowing rate was 1.5 mL/min.RESULTS Menthol,matrine,sophocarpine and sophoridine showed good linear relationships within the ranges of 0.228 7-2.287 0 mg/mL (r =0.999 7),0.011 1-0.111 0 mg/mL (r =0.999 6),0.016 8-0.168 0 mg/mL (r =0.999 8) and 0.003 4-0.034 0 mg/mL (r =0.999 4),whose average recoveries (n =9) were 98.4％ (RSD=1.2％),95.9％ (RSD=1.8％),96.2％ (RSD=2.1％) and 97.1％ (RSD=1.7％),respectively.CONCLUSION This accurate,sensitive and simple method can be used for the quality control of Kaihoujian Spray.
