Establishment of HPLC fingerprints of Qingfeiyucuo Pills and determination of four constituents
10.3969/j.issn.1001-1528.2017.01.017
- VernacularTitle:清肺愈痤丸HPLC指纹图谱建立及4种成分测定
- Author:
Shao-Mei LIANG
1
;
Xin ZHANG
;
Min-Ting LI
;
Xing-Xing CHEN
;
Li-Yan DONG
Author Information
1. 南方医科大学中西医结合医院
- Keywords:
Qingfeiyucuo Pills;
HPLC fingerprints;
paeoniflorin;
hesperidin;
baicalin;
salvianolic acid B
- From:
Chinese Traditional Patent Medicine
2017;39(1):90-93
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the HPLC fingerprints of Qingfeiyucuo Pills (an agent for the management of acne,containing Scutellariae Radix,Eriobotryae Folium,Paeoniae Radix Rubra,etc.) and to determine the contents of four constituents.METHODS The analysis of methanol extract of Qingfeiyucuo Pills was carried out on a 35 ℃ thermostatic Agilent HC-C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.4％ phosphoric acid flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelength was set at 230 nm.RESULTS Eleven common peaks with the similarities of more than 0.9 were observed in the HPLC fingerprints of ten batches of samples.Paeoniflorin,hesperidin,baicalin and salvianolic acid B showed good linear relationships within the ranges of 0.08-0.25 μg,0.07-0.22 μg,0.2-0.60 μg and 0.06-0.18 μg,whose average recoveries were 98.7％,96.9％,97.4％ and 98.2％ with the RSDs of 0.98％,1.09％,0.82％ and 1.66％,respectively.CONCLUSION This sensitive and specific method can be used for the quality control of Qingfeiyucuo Pills.
