Expression of dishevelled-2 in cartilage of rheumatoid arthritis and its effect on cartilage destruction
10.3760/cma.j.issn.0578-1426.2018.09.010
- VernacularTitle:巢乱蛋白2在类风湿关节炎软骨中的表达及其对软骨破坏的影响
- Author:
Xingzhen LIU
1
,
2
;
Ying GAO
;
Ke QI
;
Dongbao ZHAO
Author Information
1. 200433上海,海军军医大学附属长海医院风湿免疫科
2. 解放军杭州疗养院直属院区,杭州,310007
- Keywords:
Arthritis,rheumatoid;
Dishevelled 2;
Wnt signaling pathway;
Chondrocytes
- From:
Chinese Journal of Internal Medicine
2018;57(9):674-678
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of dishevelled-2 (DVL2) in rheumatoid arthritis cartilage and its effect on cartilage destruction.Methods Cartilage DVL2 expression in rat models of rheumatoid arthritis (RA),osteoarthritis(OA) and collagen-induced arthritis(CIA) were tested by Western blotting.DVL2 overexpressed lentivirus was transfected into the knee of CIA rats.Primary chondrocytes were extracted from RA patients by knee arthroplasty and transfected with DVL2 overexpressed lentivirus.Gene expression of related inflammation related cytokines was detected by real-time polymerase chain reaction (PCR).Results Compared with knee articular cartilage in OA patients and normal rats,DVL2 protein was highly expressed in knee cartilage of RA patients and CIA rats (P values 0.041 and 0.032,respectively).DVL2 did not significantly affect the destruction of knee cartilage in CIA rats (P=0.885).DVL2 overexpression in chondrocytes enhanced gene expression of cyclo-oxygenase-2 (COX-2),inducible nitric oxide synthase (NOS),matrix metalloproteinase (MMP) 2,MMP-3,and MMP-9,which could be more pronounced when tumor necrosis factor alpha was added.Conclusions DVL2 is highly expressed in RA articular cartilage and promotes the expression of inflammatory cytokines and MMP gene in chondrocytes by activating Wnt/β-catenin pathway,which involves in the destruction of articular cartilage in RA.
