Study of betulinic acid to improve non-alcoholic fatty liver disease
10.3760/cma.j.issn.1000-6699.2018.11.009
- VernacularTitle:桦木酸改善非酒精性脂肪肝的研究
- Author:
Lei TONG
1
;
Long WANG
;
Lina JIN
;
Shuangshuang YAO
;
Jian YANG
;
Zhiguo ZHANG
Author Information
1. 200025 上海交通大学医学院附属瑞金医院内分泌代谢病科,上海市内分泌代谢病研究所,上海市内分泌代谢病临床医学中心
- Keywords:
High-fat diet;
Obese mice;
Non-alcoholic fatty liver disease;
Fatty acid synthase
- From:
Chinese Journal of Endocrinology and Metabolism
2018;34(11):939-945
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of betulinic acid on NAFLD and its mechanism. Methods We used the high-fat diet animal models, with or without feeding the standard chow diet containing betulinic acid for 2 months. During this period, the body weight was monitored regularly and metabolism cage was used to monitor the energy metabolism of the animals. After killing the mice, molecular biological analysis was performed on serum and tissue related to liver. Results In diet induced obese mice animal experiments, the mice body weight had been reduced and NAFLD had been improved significantly by betulinic acid. The various indexs of serum and liver tissue had also been significantly improved. The metabolic rate increased significantly. Fatty acid synthase gene and protein levels were significantly lower. Furthermore, FAS activity was significantly lower than the control mice. Liver FAS activity of the high fat mice and the high fat mice treated with betulinic acid were (1873.6 ± 85.7) and (1181.6 ± 85.7) pmol NADPH/ min/ mg protein, respectively. Conclusion Betulinic acid inhibited FAS at expression and activity level, and improved lipid deposition in liver.
