Experimental study of regulatory effect of hydrogen sulfide on sFlt-1 production in adipocytes through ADAM17
10.3760/cma.j.issn.1000-6699.2018.09.013
- VernacularTitle:硫化氢通过ADAM17调节脂肪细胞中sFlt-1生成的实验研究
- Author:
Tianxiao HU
1
;
Gang WANG
;
Qingying TAN
;
Yun RUAN
;
Xiujing WANG
;
Jiaqi YAO
;
Yao XU
;
Jing WANG
Author Information
1. 解放军第一一七医院内分泌科
- Keywords:
Obesity;
Adipocytes;
Hydrogen sulfide;
ADAM17;
sFlt-1
- From:
Chinese Journal of Endocrinology and Metabolism
2018;34(9):784-789
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of hydrogen sulfide on the production of soluble fms-like tyrosine kinase 1 (sFlt-1) through a distintegrin and metalloproteinase 17 (ADAM17) in adipocytes. Methods 3T3-L1 cells were cultured and induced to differentiate into adipocytes, then treated with different doses of sodium hydrogen sulfide (NaHS), L-cysteine or transfected with cystathionine-γ-lyase ( CSE) siRNA, ADAM17 siRNA or treated with ADAM17 inhibitor, monoclonal antibody. 24 hours after treatment, the expression of ADAM17, CSE, and the production of sFlt-1 were determined. Results After the treatment of 10, 25, 50 nmol/L NaHS or 0. 5, 1. 0, 2. 0 μmol/L L-cysteine, the expression of ADAM17 and the production of sFlt-1 in adipocytes were significantly decreased, the higher dose of L-cysteine and sFlt-1, the lower expression of ADAM17 and the production of sFlt-1; the effect of 2.0 μmol/L L-cysteine decreasing the expression of ADAM17 and the production of sFlt-1 were reversed by transfection of CSE siRNA; after the transfection of ADAM17 siRNA and treatment of ADAM17 inhibitor or monoclonal antibody, the production of sFlt-1 in adipocytes were significantly decreased. Conclusion Hydrogen sulfide can reduce the production of sFlt-1 in adipocytes by downregulating the expression of ADAM17.
