Qualitative assessment of washed human normal sperm after cryopreservation
- Author:
Tien Phuong Thao Nguyen
;
Son Khang Nguyen
- Publication Type:Journal Article
- Keywords:
Washed sperm;
sperm cryopreservation
- MeSH:
Washed sperm;
sperm cryopreservation
- From:Journal of Medical Research
2008;55(3):37-41
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: Sperm cryopreservation becomes a relatively routine process in assisted reproductive centers. However, there must be ensured quality of washed human normal sperm and cryopreservation to successful fertilization. Objective: To evaluate the quality of washed human normal sperm after cryopreservation. Subjects and method: 30 normal semen samples, each sample was divided into two parts for washed and unwashed spermatozoa. All samples were cryopreserved in 1, 2 and 30 days. Evaluating and comparing the quality of sperm before and after which washed, pre-cryopreservation and post-cryopreservation between the groups were performed. Results: The quality of sperm after washing was more significantly improved than before washing. Post-cryopreservation, the quality of sperm was reduced time by time but within an accepted limitation. There was not a significant difference between the two ways of preparation before cryopreservation. Conclusions: The quality of sperm at post-cryopreservation was reduced (both washed sperm and unwashed sperm). The quality of washed sperm is reduced continuously with time, but there was no difference between the two studied groups.
