Role of LncRNA ROR in Ad36-induced browning of human adipose-derived stem cells
10.3760/cma.j.issn.1000-6699.2018.04.010
- VernacularTitle:LncRNA ROR对Ad36诱导人脂肪源性干细胞棕色化的作用
- Author:
Ling LIU
1
;
Yi JIAO
;
Xiaodi LIANG
;
Jianfei LU
;
Dan ZHANG
;
Shuwen ZHANG
;
Nuermaimaiti NURBIERYE
;
Xuanyu MENG
;
Jie LIU
;
Tingting HU
;
Yaqun GUAN
Author Information
1. 新疆医科大学基础医学院生化教研室
- Keywords:
Human adipose-derived stem cells;
Browning;
Cocktail;
Ad36;
Long noncoding RNA
- From:
Chinese Journal of Endocrinology and Metabolism
2018;34(4):318-324
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of LncRNA ROR in Ad36-induced browning of human adipose-derived stem cells(hADSC). Methods After hADSC was induced by cocktail and Ad36 for 2,4,6,and 8 days,Oil red O staining was performed for observing the adipogenic status. The mRNA expressions of LncRNA ROR, uncoupling protein 1(UCP1),and PRDM16 were detected by real-time PCR and the protein expressions of UCP1 and PRDM16 were detected by Western-blot. After LncRNA ROR was knocked down by siRNA, UCP1 and PRDM16 mRNA and protein expression levels in the process of Ad36-induced adipocyte differentiation were detected by real-time PCR and Western-blot. Results Oil red O staining showed that fat droplets in the cocktail-induced group were larger than those in the Ad36-induced group. Compared with the cocktail group,the mRNA expressions of LncRNA ROR, UCP1 and PRDM16, and the protein expressions of UCP1 and PRDM16 in Ad36 group were significantly increased(P<0.05). The mRNA and protein expressions of UCP1 and PRDM16 in LncRNA ROR knockdown group were significantly lower than those in control group(P<0.05). Conclusion In the process of Ad36-induced hADSC differentiation,the up-regulation of LncRNA ROR may stimulate UCP1 and PRDM16 expression,and thus promote the browning of hADSC.
