The mechanism of bone marrow-derived endothelial progenitor cells in the treatment of peripheral neuropathy of diabetic rats
10.3760/cma.j.issn.1000-6699.2018.04.009
- VernacularTitle:骨髓内皮祖细胞移植治疗糖尿病大鼠周围神经病变的机制探讨
- Author:
Rongrong YUAN
1
;
Yu ZHANG
;
Dandan LI
;
Liping ZHU
;
Wei GAO
;
Lei XUE
;
Wuyan PANG
;
Zhigang ZHAO
Author Information
1. 河南大学淮河医院内分泌科
- Keywords:
Endothelial progenitor cells;
Diabetic rats;
Diabetic peripheral neuropathy
- From:
Chinese Journal of Endocrinology and Metabolism
2018;34(4):312-317
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of bone marrow-derived endothelial progenitor cells (EPCs)in the treatment of peripheral neuropathy of diabetic rats. Methods Rats with diabetic peripheral neuropathy (DPN)were induced by streptozotocin(60 mg/kg). Male SD rats were divided into normal control group(NC group),DPN group, DPN+saline group(DPN+S group), and DPN+ EPCs group. Sciatic nerve motor nerve conduction velocity(MNCV)was measured. The expressions of NF-κB and myelin basic protein(MBP)in sciatic nerve were detected by immunohistochemistry. Results Compared with DPN group,the expression of NF-κB was reduced in the sciatic nerve of DPN+EPCs group,while the expression of NF-κB was increased in the sciatic nerve of DPN+ S group. There was no statistical difference in the expression of MBP between DPN and DPN+ EPCs groups. Compared with DPN+S group, the expression of MBP was higher in DPN+EPCs group. Conclusion Transplantation of EPCs inhibits the expression of NF-κB and increases the expression of MBP, which might be conducive to the repairs of nerve injury.
