Effects of short-chain fatty acids on oxidative stress and inflammation in glomerular mesangial cells induced by high glucose
10.3760/cma.j.issn.1000-6699.2018.03.010
- VernacularTitle:短链脂肪酸对高糖诱导的肾系膜细胞氧化应激-炎症的影响
- Author:
Wei HUANG
1
;
Luping ZHOU
;
Yong XU
;
Youhua XU
;
Yang LONG
;
Chenlin GAO
Author Information
1. 西南医科大学附属医院内分泌科
- Keywords:
Short-chain fatty acids;
Oxidative stress;
Inflammation;
Diabetic kidney disease
- From:
Chinese Journal of Endocrinology and Metabolism
2018;34(3):239-245
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of short-chain fatty acids(SCFAs)on oxidative stress and inflammation in cultured glomerular mesangial cells(GMCs)under high glucose.SV-40 MES 13 mouse GMCs were exposed to 30 mmol/L glucose, exogenous SCFAs or their specific G-protein coupled receptor 43(GPR43)agonist were used individually as an intervention.GPR43 expression was suppressed by transfection with GPR43-specifc siRNA.The oxidative stress-related factors reactive oxygen species and malondialdehyde were detected and the expression of GPR43,monocyte chemotactic protein 1(MCP-1),and interleukin-1β(IL-1β)were observed.GPR43 expression were significantly down-regulated,but reactive oxygen species and malondialdehyde production and MCP-1 and IL-1β releases were induced by high glucose(all P<0.05),treatment with SCFAs or GPR43 agonist reversed high glucose-induced oxidative stress and inflammation in a dose-dependent manner(all P<0.05).However, these SCFAs-mediated effects were blunted by siRNA-mediated knockdown GPR43(all P<0.05).SCFAs mitigates high glucose-induced oxidative stress and inflammatory injury in part via GPR43 signaling pathway,suggesting a likely mechanism for SCFAs-induced therapeutic effect in diabetic kidney disease.
