Improve diagnostic performance of PCOS by measurements of testosterone and dehydcoepiandrosterone sulfate with ultra-performance liquid chromatography tandem mass spectrometry
10.3760/cma.j.issn.1000-6699.2018.02.008
- VernacularTitle:超高压液相色谱-质谱联用技术联合分析血清睾酮和硫酸脱氢表雄酮提高多囊卵巢综合征的诊断率
- Author:
Xi XIA
1
;
Yuying DENG
;
Junjie GU
;
Yanyun GU
;
Yifei ZHANG
;
Xuemei TONG
;
Wenzhong ZHOU
Author Information
1. 上海市肿瘤微环境与炎症重点实验室
- Keywords:
Polycystic ovarian syndrome;
Ultra-performance liquid chromatography tandem mass spectrometry;
Chemiluminescence immunoassay;
Testosterone;
Dehydroepiandrosterone sulfate;
Area under curve
- From:
Chinese Journal of Endocrinology and Metabolism
2018;34(2):136-140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) and chemiluminescence immunoassay(CLIA) measurement of human serum androgen levels in polycystic ovarian syndrome(PCOS). Methods 160 PCOS patients and 146 healthy subjects(control group) were recruited and their serum androgen levels——measurements of testosterone and dehydcoepiandrosterone sulfate (DHEA-S) were tested by UPLC-MS/MS and CLIA. The androgen results combined with body mass index(BMI) were analyzed by ROC curve. According to area under curve(AUC) calculated by SPSS, a better method will be selected to provide accurate test results for physicians. Results (1)AUC of DHEA-S tested by UPLC-MS/MS was significantly higher than the one tested by CLIA(P<0.01). There was no significant difference between the AUC of T tested by UPLC-MS/MS and the one tested by CLIA. (2)AUC of T combined with DHEA-S tested by HPLC was not only significantly higher than the AUC of two combined indicators tested by CLIA(P<0.01),but also significantly higher than the AUC of a single indicator——either T(P<0.01) or DHEA-S(P<0.01) tested by UPLC-MS/MS. (3)The AUC of T,DHEA-S combined with BMI tested by HPLC was not only significantly higher than the AUC of three combined indicators tested by CLIA(P<0.01),but also higher than the AUC of two combined indicators tested by UPLC-MS/MS(P<0.05). Conclusion T and DHEA-S tested by UPLC-MS/MS combined with BMI has a certain reference value in the diagnosis of PCOS.
