Effect of sevoflurane preconditioning on expression of metabotropic glutamate receptor typeⅡ dur-ing focal cerebral ischemia-reperfusion in rats
10.3760∕cma.j.issn.0254-1416.2018.07.028
- VernacularTitle:七氟醚预处理对大鼠局灶性脑缺血再灌注时mGluRⅡ表达的影响
- Author:
Zheng ZHU
1
;
Meiyan SUN
;
Rui ZHANG
;
Danyang MA
;
Xiaoli ZHUANG
;
Yan LI
;
Du ZHENG
;
Zhaodong JUAN
;
Xiaoyong ZHAO
Author Information
1. 261053,潍坊医学院山东省医药卫生临床麻醉重点实验室
- Keywords:
Anesthetics,inhalation;
Ischemic preconditioning;
Brain;
Reperfusion injury;
Receptors,glutamate
- From:
Chinese Journal of Anesthesiology
2018;38(7):882-885
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of sevoflurane preconditioning on the expression of metabotropic glutamate receptor type Ⅱ( mGluRⅡ) during focal cerebral ischemia-reperfusion ( I∕R) in rats. Methods Forty-eight clean-grade healthy male Sprague-Dawley rats were divided into 3 groups (n＝16 each) using a random number table method: sham operation group ( group S), cerebral I∕R group (group I∕R) and sevoflurane preconditioning group (group Sev). Rats were anesthetized with 10% chloral hydrate 3 ml∕kg. Focal cerebral I∕R was produced by occlusion of the right middle cerebral artery for 2 h fol-lowed by 24 h reperfusion. In group Sev, 2. 7% sevoflurane was inhaled for 1 h and 24 h later focal cerebral I∕R was produced. At 24 h after reperfusion, neurological deficit was scored, the cerebral infarct size was determined by TTC staining, the cell apoptosis in ischemic penumbra was observed by TUNEL, IκB-α ex-pression was detected by Western blot, and mGluRⅡexpression was determined by immunofluorescent stai-ning. The apoptosis rate was calculated. Results Compared with group S, the neurological deficit score, cerebral infarct size and apoptosis rate were significantly increased, the expression of mGluRⅡwas up-regu-lated, and the expression of IκB-α was down-regulated in I∕R and Sev groups ( P＜0. 05). Compared with group I∕R, the neurological deficit score, cerebral infarct size and apoptosis rate were significantly de-creased, the expression of mGluRⅡwas down-regulated, and the expression of IκB-α was up-regulated in group Sev (P＜0. 05). Conclusion Sevoflurane preconditioning reduces focal cerebral I∕R injury through inhibiting the expression of mGluRⅡ in rats.
