Effect of sevoflurane-containing HTK solution on electrophysiological stability of rat donor heart during reperfusion
10.3760∕cma.j.issn.0254-1416.2018.07.014
- VernacularTitle:含七氟醚HTK液对大鼠供体心脏再灌注时电生理稳定性的影响
- Author:
Weichao LI
1
;
Hong GAO
;
Ju GAO
;
Yuqi SHE
;
Zijun WANG
;
Yanqiu LIU
Author Information
1. 贵州医科大学麻醉学院
- Keywords:
Anesthetics,inhalation;
Heart transplantation;
Cardiac electrophysiology
- From:
Chinese Journal of Anesthesiology
2018;38(7):825-828
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of sevoflurane-containing HTK solution on electro-physiological stability of rat donor heart during reperfusion. Methods Male Sprague-Dawley rats, weighing 280-320 g, aged 3 months, in which a Langendorff-perfused isolated rat donor heart model was estab-lished, were used in this study. Sixteen donor hearts were obtained and divided into 2 groups (n＝8 each) using a random number table method: control group (group C) and sevoflurane group (group S). HTK so-lution was used as preservation solution in group C. HTK solution containing 2. 5% sevoflurane was used as preservation solution in group S. Hearts were stored for 6 h in the corresponding preservation solution at 4℃ and then reperfused, and the perfusion temperature was gradually restored to 37 ℃ in two groups. The time of spontaneous recovery of heart beat and development of arrhythmia were recorded. Heart rate, monophasic action potential (MAP) duration at 50% and 90% repolarization (MAPD50, MAPD90), MAP amplitude and maximum velocity and development of early after-depolarization and delayed after-depolariza-tion were recorded at 30 and 45 min of reperfusion. Results Compared with group C, the time of sponta-neous recovery of heart beat was significantly shortened, the incidence of ventricular fibrillation and arrhyth- mia score were decreased, the ventricular fibrillation interval was shortened, and MAPD50and MAPD90of epicardium and endocardium were shortened at 30 and 45 min of reperfusion (P＜0. 05), and no significant change was found in hear rate, maximum velocity or MAP amplitude in group S (P＞0. 05). Early after-de-polarization and delayed after-depolarization were not found in two groups. Conclusion Sevoflurane-contai-ning HTK solution can maintain electrophysiological stability of rat donor heart during reperfusion and de-crease the occurrence of arrhythmia.
