Changes in FUNDC1/LC3 Ⅱ signaling pathway during sepsis-induced liver injury in mice
10.3760/cma.j.issn.0254-1416.2018.06.025
- VernacularTitle:脓毒症小鼠肝损伤时FUNDC1/LC3 Ⅱ信号通路的变化
- Author:
Mengying YAN
1
;
Yanyan WANG
;
Xing MAO
;
Jingcheng FENG
;
Guolin WANG
;
Keliang XIE
;
Yonghao YU
Author Information
1. 300052,天津医科大学总医院麻醉科天津市麻醉学研究所
- Keywords:
Sepsis;
Liver;
Membrane proteins;
Autophagy
- From:
Chinese Journal of Anesthesiology
2018;38(6):736-738
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in FUNDC1/microtubule-associated protein 1 light chain 3 Ⅱ (LC3 Ⅱ) signaling pathway during sepsis-induced liver injury in mice.Methods Tbirtytwo clean-grade healthy male C57BL/6 mice,aged 6 weeks,weighing 20-25 g,were divided into sham operation group (n =8) and sepsis group (n =24) using a random number table method.Sepsis was induced by cecal ligation and puncture.Blood samples were obtained at 24 h after operation in sham operation group and at 6,12 and 24 h after establishing the model in sepsis group for determination of concentrations of alanine aminotransferase and aspartate aminotransferase in serum.Mice were then sacrificed,and the right lobe of livers was removed for examination of the pathological changes and for determination of the expression of FUNDC1 and LC3 Ⅱ by Western blot.The mitochondria in the right lobe of livers were isolated to measure the respiratory function,and respiratory control rate was calculated.Results Compared with sham operation group,the concentrations of alanine aminotransferase and aspartate aminotransferase in serum and pathological scores were significantly increased,the respiratory control rate of mitochondria was decreased,the expression of FUNDC1 was down-regulated,and the expression of LC3 Ⅱ was up-regulated at each time point after establishing the model in sepsis group (P＜0.05).Conclusion The mechanism by which sepsis induces liver injury may be related to inhibiting activation of FUNDC1/LC3 Ⅱ signaling pathway in mice.
