Effect of dexmedetomidine on mitochondrion-dependent apoptosis during hypoxia-reoxygenation injury to hippocampal neurons of rats
10.3760/cma.j.issn.0254-1416.2018.06.005
- VernacularTitle:右美托咪定对大鼠海马神经元缺氧复氧损伤时线粒体途径凋亡的影响
- Author:
Jia LIU
1
;
Lantao ZHAO
;
Shaona LI
;
Lixiao PAN
;
Huijuan SUN
;
Fengyun YANG
;
Shilei WANG
Author Information
1. 266555,青岛大学附属医院麻醉科
- Keywords:
Dexmedetomidine;
Anoxia;
Mitochondria;
Hippocampus;
Neurons
- From:
Chinese Journal of Anesthesiology
2018;38(6):656-659
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate effect of dexmedetomidine on mitochondrion-dependent apoptosis during hypoxia-reoxygenation (H/R) injury to hippocampal neurons of rats.Methods The primarily cultured hippocampal neurons of Sprague-Dawley rats were divided into 4 groups (n =40 each) using a random number table method:control group (C group),vehicle group (V group),H/R group and dexmedetomidine group (D group).Hippocampal neurons were subjected to oxygen-glucose deprivation followed by restoration of oxygen supply to establish the model of H/R injury.Dexmedetomidine 1 μmol/L was added at 6 h of reoxygenation in D group.The viability of neurons was measured by methyl thiazolyl tetrazolium assay at 20 h of reoxygenation.The ultrastructure of mitochondria was observed by transmission electron microscopy.The expression of cytochrome c (Cyt c),caspase-3,Fis1 and Drp1 was detected by Western blot.The neuronal apoptosis was detected by flow cytometry,and apoptosis rate was calculated.Results Compared with C group,no significant change was found in the viability of neurons in group V (P＞0.05),and the viability of neurons was significantly decreased,the apoptosis rate was increased,the expression of Cyt c,caspase-3,Fis1 and Drp1 was up-regulated (P＜0.05),and the damage to mitochondrial ultrastructure was accentuated in H/R and D groups.Compared with H/R group,the viability of neurons was significantly increased,the apoptosis rate was decreased,the expression of Cyt c,caspase-3,Fis1 and Drp1 was down-regulated (P＜0.05),and the damage to mitochondrial ultrastructure was significantly attenuated in D group.Conclusion The nechanism by which dexmedetomidine reduces the H/R injury to hippocampal neurons is related to inhibiting mitochondrion-dependent apoptosis in rats.
