Changes in expression of cold-inducible RNA-binding protein in hippocampus during brain injury in a rat model of cardiac arrest-cardiopulmonary resuscitation
10.3760/cma.j.issn.0254-1416.2018.05.025
- VernacularTitle:心搏骤停/心肺复苏大鼠脑损伤时海马冷诱导RNA结合蛋白表达的变化
- Author:
Yongyi CHEN
1
;
Manlin DUAN
;
Minhao ZHANG
;
Liangbin CAO
;
Juan LI
;
Jiejie ZHOU
;
Lianbing GU
Author Information
1. 210009,江苏省肿瘤医院 江苏省肿瘤防治研究所 南京医科大学附属肿瘤医院
- Keywords:
RNA-binding protein;
Heart arrest;
Cardiopulmonary resuscitation;
Brain injuries
- From:
Chinese Journal of Anesthesiology
2018;38(5):602-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the changes in expression of cold-inducible RNA-binding protein (CIRP) in hippocampus during brain injury in a rat model of cardiac arrest-cardiopulmonary resuscitation.Methods Seventv-two clean-grade healthy male Sprague-Dawley rats,weighing 280-350 g,aged 8-10 weeks,were divided into 2 groups using a random number table:sham operation group (S group,n=18) and ischemia-reperfusion group (I/R group,n=54).Tracheal intubation was performed and artery and veins were punctured in group S.Ventricular fibrillation was induced by transoesophageal cardiac pacing to establish the model of cardiac arrest in group I/R.Rats were sacrificed at 12,24 and 48 h after resuscitation and the hippocampus was harvested for determination of CIRP,tumor necrosis factor-alpha (TNF-α) and interleukin-lbeta (IL-1β) protein and mRNA expression (by quantitative polymerase chain reaction or Western blot) and for determination of pathological changes of hippocampi (with a light microscope).Results Compared with group S,the expression of CIRP mRNA in hippocampus was up-regulated at 24 and 48 h after resuscitation,the expression of TNF-α mRNA was up-regulated at 12,24 and 48 h after resuscitation,the expression of IL-1β mRNA was up-regulated at 12 and 24 h after resuscitation,and the expression of CIRP,TNF-α and IL-1β was up-regulated at 12,24 and 48 h after resuscitation in group I/R (P＜0.05).Pathological changes in hippocampal CA1 region were found in group I/R.Conclusion The expression of CIRP in hippocampus is up-regulated,which promotes central inflammatory responses during brain injury in a rat model of cardiac arrest-cardiopulmonary resuscitation.
