Effects of peiminine preconditioning on ventilator-induced lung injury in mice
10.3760/cma.j.issn.0254-1416.2018.04.032
- VernacularTitle:贝母素乙预处理对小鼠机械通气相关性肺损伤的影响
- Author:
Jingjing WANG
1
;
Yulan LI
;
Qian TONG
;
Xiaofang MA
;
Rui WANG
;
Qiang JIA
Author Information
1. 730000,兰州大学第一临床医学院
- Keywords:
Fritillaria;
Respiration,artificial;
Respiratory distress syndrome,adult
- From:
Chinese Journal of Anesthesiology
2018;38(4):505-508
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of peiminine preconditioning on ventilator-induced lung injury (VILI) in mice.Methods Thirty-two clean healthy male BALB/c mice,aged 9-10 weeks,weighing 20-25 g,were divided into 4 groups (n=8 each) using a random number table:control group (group C),VILI group,dexamethasone group (group D) and peiminine preconditioning group (group P).Normal saline 0.2 ml was administered by intragastric gavage for 7 days in C and VILI groups,dexamethasone 10 mg/kg was administered by intragastric gavage for 7 days in group D,and peiminine 1 mg/kg was administered by intragastric gavage for 7 days in group P.VILI model (respiratory rate 80 breaths/min,tidal volume 40 ml/kg,fraction of inspired oxygen 21％,inspiratory/expiratory ratio 1 ∶ 2,for 4 h) was established on the 7th day of intragastric administration.After the end of ventilation,blood samples were collected from hearts for determination of serum interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) concentrations (by enzyme-linked immunosorbent assay),and bilateral lung tissues were obtained for examination of the pathological changes after haematoxylin and eosin staining and for determination of contents of aquaporin 1 (AQP-1) and AQP-5 in lung tissues.Results Compared with group C,the concentrations of IL-8 and TNF-α in serum were significantly increased and the contents of AQP-1 and AQP-5 in lung tissues were decreased in group VILI (P＜0.05),and no significant change was found in the parameters mentioned above in D and P groups (P＞0.05).Compared with group VILI,the concentrations of IL-8 and TNF-α in serum were significantly decreased,the content of AQP-1 in lung tissues was increased (P＜0.05),no significant change was found in AQP-1 content (P＞0.05),and the pathological changes were significantly attenuated in D and P groups.Compared with group D,no significant change was found in concentrations of IL-8 and TNF-α in serum or contents of AQP-1 and AQP-5 in lung tissues (P＞0.05),and the pathological changes were comparable in group P.Conclusion Peiminine preconditioning can reduce VILI in mice.
