Effect of ulinastatin on postoperative cognitive dysfunction in preoperative sleep deprived aged rats
10.3760/cma.j.issn.0254-1416.2018.04.012
- VernacularTitle:乌司他丁对术前睡眠剥夺老龄大鼠术后认知功能障碍的影响
- Author:
Xiaopeng WANG
1
;
Jiandong HE
;
Weihao LUO
;
Jian CUI
;
Wenqu YANG
;
Yajuan LEI
;
Ping ZHUANG
;
Chongfang HAN
Author Information
1. 山西医学科学院 山西大医院麻醉科
- Keywords:
Trypsin inhibitors;
Sleep deprivation;
Aged;
Cognition disorders
- From:
Chinese Journal of Anesthesiology
2018;38(4):428-430
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of ulinastatin on postoperative cognitive dysfunction in preoperative sleep deprived aged rats.Methods Sixty clean healthy male Sprague-Dawley rats,aged 18 months,weighing 350-500 g,were divided into 3 groups (n=20 each) using a random number table:control group (group C),sleep deprivation group (group D) and ulinastatin group (group U).Sleep deprivation was induced by using modified multiple platform method in D and U groups,and then splenectomy was performed in three groups.Ulinastatin 100 U/g was intraperitoneally injected before sleep deprivation and immediately after operation in group U.Ten rats were randomly selected at 3 days after operation and sacrificed,and hippocampi were removed for determination of the contents of interleukin-1β (IL-1β),IL-6 and tumor necrosis factor-α (TNF-α) by enzyme-linked immunosorbent assay.Morris water maze test was performed at 3-7 days after operation in the rest ten rats in each group,and the escape latency and time of staying at the original platform were recorded.Results Compared with group C,the escape latency was significantly prolonged at 4-6 days after operation,the time of staying at the original platform was shortened,and the contents of IL-1β,IL-6 and TNF-α were increased at 3 days after operation in D and U groups (P＜0.05).Compared with group D,the escape latency was significantly shortened at 4-6 days after operation,the time spent in the original platform was prolonged,and the contents of IL-1β,IL-6 and TNF-α were decreased at 3 days after operation in group U (P＜0.05).Conclusion Ulinastatin can mitigate postoperative cognitive dysfunction in preoperative sleep deprived aged rats,which is related to inhibiting inflammatory responses.
