Effect of dexmedetomidine on HMGB1/TLRs signaling pathway during lung injury in septic rats
10.3760/cma.j.issn.0254-1416.2018.02.029
- VernacularTitle:右美托咪定对脓毒症大鼠肺损伤时HMGB1/TLRs信号通路的影响
- Author:
Lin ZHANG
1
;
Jiaqiang ZHANG
;
Fanmin MENG
;
Hongfang GENG
;
Lidong DOU
;
Chunyan WU
Author Information
1. 河南省人民医院麻醉科
- Keywords:
Dexmedetomidine;
Sepsis;
Respiratory distress syndrome,adult;
High mobility group proteins;
Toll-like receptors
- From:
Chinese Journal of Anesthesiology
2018;38(2):238-241
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of dexmedetomidine on high-mobility group box 1 protein (HMGB1)/Toll-like receptors (TLRs) signaling pathway during lung injury in septic rats.Methods Twenty-four SPF healthy adult male Wistar rats,aged 15-18 weeks,weighing 200-250 g,were divided into 3 groups (n =8 each) using a random number table:sham operation group (group S),sepsis group (group Sep) and dexmedetomidine group (group D).Dexmedetomidine 25 μg/kg was intraperitoneally injected in D group,while the equal volume of normal saline was given instead in S and Sep groups.Sepsis was produced by cecal ligation and puncture in Sep and D groups.The rats were sacrificed at 24 h after operation,and the right lung was removed for examination of the pathological changes which were scored and for determination of myeloperoxidase (MPO) activity,content of interleukin-1β (IL-1β),IL-6 and tumor necrosis factor-α (TNF-α) in lung tissues (by enzyme-linked immunosorbent assay),wet to dry weight ratio (W/D ratio) and expression of HMGB1,TLR2 and TLR4 in lung tissues (by Western blot).Results Compared with group S,the MPO activity,lung injury score,W/D ratio,content of IL-1β,IL-6,TNF-α and expression of HMGB1,TLR2 and TLR4 were significantly increased in Sep and D groups (P＜0.05).Compared with group Sep,the MPO activity,lung injury score,W/D ratio,content of IL-1β,IL-6,TNF-α and expression of HMGB1,TLR2 and TLR4 were significantly decreased in group D (P＜0.05).Conclusion Dexmedetomidine reduces lung injury through inhibiting HMGB1/TLRs signaling pathway in septic rats.
