Effect of electroacupuncture preconditioning on IRE1-XBP1 signaling pathway in endoplasmic reticulum in cortex in a rat model of cerebral ischemia-reperfusion
10.3760/cma.j.issn.0254-1416.2018.02.026
- VernacularTitle:电针预处理对脑缺血再灌注大鼠皮层内质网IRE1-XBP1信号通路的影响
- Author:
Fei XIAO
1
;
Gaofeng ZHANG
;
Zeng YIN
;
Huailong CHEN
;
Fuguo MA
;
Zhaoliang ZHANG
;
Mingshan WANG
;
Fei SHI
Author Information
1. 266071,青岛市市立医院麻醉科
- Keywords:
Electric stimulation therapy;
Cerebral ischemia;
Reperfusion injury;
Endoplasmic reticulum;
Stress
- From:
Chinese Journal of Anesthesiology
2018;38(2):226-229
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of electroacupuncture (EA) preconditioning on inositol-requiring kinase 1 (IRE1)-X-box binding protein 1 (XBP1) signaling pathway in endoplasmic reticulum in the cortex in a rat model of cerebral ischemia-reperfusion (I/R).Methods One hundred and eight pathogen-free healthy male Sprague-Dawley rats,aged 8-12 weeks,weighing 200-250 g,were assigned into 3 groups (n =36 each) using a random number table:sham operation group (group S),group I/R and EA preconditioning group (group EA).Focal cerebral I/R was induced by occlusion of right middle cerebral arteries for 2 h followed by reperfusion in rats anesthetized with chloral hydrate.In group EA,Baihui acupoints were stimulated with an electric stimulator for 30 min once a day for 5 consecutive days starting from 5 days before ischemia,and the model was established at 24 h after the last preconditioning.Rats were sacrificed after neurological deficit was scored at 6,12 and 24 h of reperfusion,brains were removed,and the ischemic area in cerebral cortex was isolated for examination of the cell ultrastructure (with an electronic microscope) and for determination of the expression of IRE1 and XBP1 (by Western blot).Results Compared with group S,the neurological deficit scores were significantly increased,and the expression of IRE1 and XBP1 in the ischemic area was up-regulated at each time point in I/R and EA groups (P＜0.01).Compared with group I/R,the neurological deficit scores were significantly decreased,and the expression of IRE1 and XBP1 was up-regulated at each time point in group EA (P＜0.05).The cell damage in the ischemic area in cerebral cortex was significantly attenuated in group EA when compared with group I/R.Conclusion The mechanism by which EA preconditioning attenuates cerebral I/R injury is related to activating IRE1-XBP 1 signaling pathway and relieving endoplasmic reticulum stress in rats.
