Role of heme oxygenase-1 in sevoflurane-induced reduction of airway injury in asthmatic mice
10.3760/cma.j.issn.0254-1416.2017.12.029
- VernacularTitle:血红素氧合酶-1在七氟醚减轻哮喘小鼠气道损伤中的作用
- Author:
Hongtao ZHANG
1
;
Jie ZHAO
;
Yonghao YU
;
Yu LIANG
;
Junhu JING
Author Information
1. 300350,天津市环湖医院麻醉科 天津市脑血管病与神经变性重点实验室
- Keywords:
Asthma;
Anesthetics,inhalation;
Heme oxygenase-1
- From:
Chinese Journal of Anesthesiology
2017;37(12):1524-1528
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of heme oxygenase-1 (HO-1) in sevoflurane-induced reduction of airway injury in asthmatic mice.Methods Forty SPF female C57BL/6 mice,aged 6-8 weeks,weighing 20-25 g,were divided into 4 groups (n=10 each) using a random number table:control group (group C),asthma group (group A),asthma plus sevoflurane group (group A+S) and asthma plus sevoflurane plus zinc protoporphyrin group (group A+S+Z).The mice were sensitized and repeatedly challenged with ovalbumin to establish the asthma model.In group A+S,3％ sevoflurane was inhaled for 1 h after every challenge.In group A+S+Z,zinc protoporphyrin 10 mg/kg was intraperitoneally injected after every challenge and before sevoflurane inhalation.The airway responsiveness were assessed at 24 h af=ter the last challenge.The bronchoalvelor lavage fluid (BALF) was collected for determination of cell type and count and concentrations of interleukin-13 (IL-13) and IL-33 (by enzyme-linked immunosorbent assay).The right lung tissues were obtained for measurement of the superoxide dismutase (SOD) activity,contents of malondialdehyde (MDA) and glutathione (GSH) and expression of HO-1 (by Western blot).Lung tissues were stained with haematoxylin and eosin and inflammatory cell infiltration was assessed and scored.Results Compared with group C,the airway responsiveness,inflammatory cell count and concentrations of IL-13 and IL-33 in BALF,and MDA content and infiltration score in lung tissues were significantly increased,the SOD activity and GSH content were decreased,and the expression of HO-1 was upregulated in group A (P＜0.05).Compared with group A,the airway responsiveness,inflammatory cell count and concentrations of IL-13 and IL-33 in BALF,and MDA content and infiltration score in lung tissues were significantly decreased,the SOD activity and GSH content were increased,and the expression of HO-1 was up-regulated in group A+S (P＜0.05),and the expression of HO-1 was down-regulated (P＜ 0.05),and no significant change was found in the other parameters mentioned above in group A+S+Z (P＞ 0.05).Compared with group A+S,the airway responsiveness,inflammatory cell count and concentrations of IL-13 and IL-33 in BALF,and MDA content and infiltration score in lung tissues were significantly increased,the SOD activity and GSH content were decreased,and the expression of HO-1 was down-regulated in group A+S+Z (P＜0.05).Conclusion HO-1 is involved in the pathophysiological mechanism by which sevoflurane attenuates airway injury in asthmatic mice.
