The protective mechanism of D-pinitol on advanced glycation end products-induced proliferation and migration in vascular smooth muscle cells
10.3760/cma.j.issn.0254-9026.2018.11.024
- VernacularTitle:D-松醇对晚期糖基化终末产物诱导的血管平滑肌细胞增殖和迁移的保护机制
- Author:
Mei XU
1
;
Fei YU
;
Chunli FU
;
Xin YU
;
Jiayan HU
;
Hong ZHANG
;
Haiqing GAO
;
Mei CHENG
Author Information
1. 250012,山东大学齐鲁医院老年病科山东省心血管蛋白质组学重点实验室
- Keywords:
D-pinitol;
Muscle,smooth,vascular;
Glycosylation end products,advanced;
Extracellular matrix
- From:
Chinese Journal of Geriatrics
2018;37(11):1283-1286
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and its mechanism of D-pinitol on advanced glycation end products(AGEs)-induced proliferation and migration in mouse aortic vascular smooth muscle cells (VSMC).Methods VSMCs were isolated from mouse aorta and cultured in vitro.Effects of different concentrations of D-pinitol on proliferation and migration of VSMCs were observed by using the AGEs-induced glycosylation injury model of VSMCs.Cell proliferation and migration were detected by CCK-8 assay and cell scratch,respectively.The protein expression levels of transforming growth factor-β1(TGF-β1),p-smad2,p-smad3 and asporin were determined by Western blot.Results Compared with control group,AGEs group showed the increased protein expression levels of asporin,TGF-β1,p-smad2 and p-smad3 (40.06 ± 4.50 vs.17.47 ± 0.57),(55.25 ± 2.07 vs.14.42± 2.07),(0.97 ± 0.02 vs.0.47 ± 0.02),(0.45±0.01 vs.0.26 ± 0.02),all P＜ 0.01.Compared to AGEs group,D-pinitol group could inhibit the cell proliferation and migration and cause dose-dependent decreases of protein expressions of TGF-β1,p-smad2,p-smad3 and asporin(P ＜ 0.05 or P＜0.01).Conclusions D-pinitol can inhibit AGEs-induced cell proliferation and migration in mouse aortic VSMCs.Asporin may participate in the VSMCs extracellular matrix remodeling via TGF-β/smad pathway.
