Effect of soluble amyloid precursor protein α on nerve cell apoptosis and neurological function in subarachnoid hemorrhage rats
10.3969/j.issn.1009-0126.2018.06.021
- VernacularTitle:可溶性α淀粉样前体蛋白对蛛网膜下腔出血大鼠神经细胞凋亡及神经功能的影响
- Author:
Haigang CHANG
1
;
Lei HUI
;
Pengju MA
;
Xiangsheng LI
;
Ruihua LIU
;
Baozhe JIN
Author Information
1. 新乡医学院第一附属医院神经外科
- Keywords:
amyloid beta-protein precursor;
subarachnoid hemorrhage;
neurons;
apoptosis;
Alzheimer disease
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2018;20(6):639-641
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of soluble amyloid precursor protein α (sAPPα) on nerve cell apoptosis and neurological function in subarachnoid hemorrhage (SAH) rats.Methods Sixty male SD rats were randomly divided into control group (n=20),SAH+saline group (n=20) and SAH+sAPPα group (n=20).A SAH model was established by injecting autologous blood into cistern magna in rats.After a SAH model was established for SAH + saline group and SAH + sAPPα group by injecting saline and sAPPα respectively into the cistern magna of rats,the apoptotic cells were detected by immunofluorescene with TUNEL staining and the neurological function was scored in 10 rats from each group on day 3 after injection of sAPPα and saline.Results The number of apoptotic cells in brain tissue was significantly greater in SAH+saline group than in control group (P＜0.05) and was significantly smaller in SAH+sAPPα group than in SAH+ saline group (P＜0.05).The neurological function score was 26.7±0.5,13.9±0.7 and 23.0±0.8 respectively in control group,SAH + saline group and SAH + sAPPα group.Conclusion sAPPα alleviates secondary damage of neurological function by inhibiting the apoptosis of nerve cells in rats after SAH and can thus improve their neurological function.
