Effect of curcumin on JAK2/STAT3 signaling pathway after cerebral ischemia in rats
10.3969/j.issn.1009-0126.2018.05.021
- VernacularTitle:姜黄素对大鼠脑缺血后Janus蛋白酪氨酸激酶2信号转导和转录激活子3信号通路的影响
- Author:
Jing XU
1
;
Yuquan WU
;
Juan XU
;
Yi DAI
;
Chun ZHOU
;
Mingfeng FEI
;
Zhan PAN
Author Information
1. 解放军第一一七医院老年病诊治中心
- Keywords:
curcumin;
brain ischemia;
Janus kinase 2;
STAT3 transcription factor;
high mobility group proteins
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2018;20(5):534-537
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of curcumin on JAK2/STAT3 signaling pathway following ischemic cerebral injury in rats.Methods Sixty-eight SD rats were divided into sham opera tion group,cerebral ischemia group,curcumin treatment group and control group (17 in each group).After a rat focal cerebral hemmorrahge model was established,the changes of neurological behaviors in rats were recorded,expressions of TNF-α,IL-1β and IL-6 were detected by ELISA and those of JAK2,p-JAK2,STAT3,p-STAT3 and HMGB1 were detected by Western blot.Results The neurological behavior score was lower in curcumin treatment group than in cerebral ischemia group (1.53±0.62 vs 2.94±0.87,P＜0.05).No significant difference was found in expression levels of TNF-α,IL-1β and IL 6 between cerebral ischemia group and control group (P＞0.05).The expression levels of TNF α,IL 1β and IL 6 were lower in curcumin treatment group than in cerebral ischemia group (57.63±10.27 ng/L vs 99.35±8.97 ng/L,33.67 ± 9.10 ng/L vs58.43±7.22 ng/L,31.97±6.91 ng/L vs 49.23±6.28 ng/L,P＜0.01).The relative volume of p-JAK2/JAK2 and p-STAT3/STAT3 and the expression level of HMGB1 were lower in curcumin treatment group than in cerebral ischemia group (P＜0.05).Conclusion Curcumin can protect rats against cerebral injury following ischemia by inhibiting the JAK2/STAT3 signaling pathway and downregulating the HMGB1 expression,and can thus alleviate inflammatory reactions.
