Effect of repetitive normobaric hypoxic preconditioning on white matter lesions and cognitive impairment in chronic cerebral ischemia rats
10.3969/j.issn.1009-0126.2018.04.020
- VernacularTitle:重复常压低氧预适应对慢性脑缺血大鼠脑白质损伤和认知功能障碍的影响
- Author:
Guoqing LI
1
;
Ran MENG
;
Changhong REN
;
Peng YU
;
Xunming JI
Author Information
1. 100054,北京市回民医院脑病科一区
- Keywords:
anoxia;
brain ischemia;
cognition disorders;
corpus callosum;
leukoencephalopathy,progressive multifocal
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2018;20(4):412-416
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of repetitive normobaric hypoxic preconditioning (RNHP) on white matter lesions (WMLs) and cognitive impairment in chronic cerebral ischemia rats.Methods Twenty-four healthy adult male SD rats were divided into sham operation group,model group,and RNHP group (8 in each group).The bilateral common carotid arteries in sham operation group were isolated but not ligated in ambient air,those in model group were ligated in ambient air,and those in RNHP group were preconditioned for 2 weeks before ligation.Their cognitive function was assessed in Morris water maze test,their WMLs were caluculated with KlüverBarrera staining.The astroglia,microglia and oligodendrocyte in cerebral white matter were stained with immunolabelling technique using antibodies to glial fibrillary acidic protein,Iba-1 and CNPase.Results The percentage of target quadrant swimming time was significantly higher in RNHP group and sham operation group than in model group (27.26％ ± 2.06％,29.06％ ± 1.72％ vs 20.58％±2.23％,P＜0.05,P＜0.01).The scores of WMLs in corpus callosum,caudate putamen and anterior commissure were significantly lower,the number of astrocytes and microglias was significantly smaller while that of oligodendrocytes was significantly greater in RNHP group and sham operation group than in model group (P＜0.05,P＜0.01).Conclusion RNHP can improve WMLs and cognitive impairment in chronic cerebral ischemia rats.
