Effect of recombinant human platelet activating factor acetylhydrolase on CPG15 expression after cerebral ischemia
10.3969/j.issn.1009-0126.2018.03.021
- VernacularTitle:重组人血小板活化因子乙酰水解酶对脑缺血后候选可塑性相关基因15蛋白的表达
- Author:
Qizheng WANG
1
,
2
;
Xiong ZHANG
;
Yumin CAO
;
Yuling XU
;
Xiaona TANG
;
Shujun FENG
;
Lijuan WANG
Author Information
1. 510080广州,广东省人民医院神经内科(广东省医学科学院)广东省神经科学研究所
2. 汕头大学医学院
- Keywords:
platelet activating factor;
brain ischemia;
infarction,middle cerebral artery;
1-alkyl-2-acetylglycerophosphocholine esterase
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2018;20(3):302-304
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of recombinant human platelet activating factor acetylhydrolase (rPAF-AH) on candidate plasticity-related gene 15 (CPG15) expression in focal cerebral ischemia rats.Methods Forty-five SD rats were randomly divided into sham operation group,saline group and rPAF-AH group (15 in each group).A middle cerebral artery occlusion model of rats was established for the latter two groups.Each group was divided into 2 d group,7 d group,14 d group.Five rats in each of 2 d group,7 d group,14 d group were used in experiment.The total protein was extracted from coronary sections using homogenate.CPG15 protein expression was detected by Western blot.Results The CPG15 protein expression level was significantly higher in saline group and rPAF-AH group than in sham operation group on days 2,7,14 and reached its peak on day 7,and in rPAF-AH group than in saline group on day 7 (1.48±0.04 vs 1.12±0.05,P＜0.01).Conclusion PAF-AH plays a positive role in neuroplasticity of cerebral ischemia by upregulating the CPG-15 expression in focal cerebral ischemia rats.
