Role of natural antiox-LDL subclass IgM antidodies in formation of atherclerosis
10.3969/j.issn.1009-0126.2018.03.020
- VernacularTitle:天然属性抗氧化型低密度脂蛋白IgM亚类抗体对动脉粥样硬化形成的机制探讨
- Author:
Xuyang FENG
1
;
Bincheng REN
;
Kaiyu CAO
;
Ruifen XU
Author Information
1. 空军军医大学西京医院心血管内科
- Keywords:
lipoproteins,LDL;
atherosclerosis;
immunoglobulin M;
macrophages;
foam cells
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2018;20(3):298-301
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of natural antiox-LDL subclass IgM antibodies in formation of atherclerosis.Methods The macrophages from mice were divded into LDL group,Cuox-LDL group and control group.The macrophages in LDL group and Cuox-LDL group were precultured with LDL and Cuox-LDL.Adhesion experiment was performed in control group.3A6,5G8 and 2H7 were incubated.The macrophages were divided into 3A6 group,5G8 group and 2H7 group.Adhesion experiment was performed by adding the pretreated 125I Cuox-LDL.The binding parameters of macrophages and 125I Cuox-LDL in different groups were recorded.Foam cells were analyzed by cell lipid analysis with oil red O staining.Results The bound 125I Cuox-LDL level was significantly lower in Cuox-LDL group than in control group and LDL group (P<0.01).No significant difference was found in bound 125I Cuox-LDL level between 5G3 group and 2H7 group (P>0.05).The bound 125I Cuox-LDL level was significantly lower in 3A6 group than in control group,5G8 group and 2H7 group (P<0.01).Oil red O staining showed no oil drop in macrophages,but a small number of oil drops in 3A6 group and a large number of oil drops in control group.The cholesterol and TG in macrophages reduced 45% and the TG reduced 170% in 3A6 group,which were significantly lower than those in control group (P<0.01).Conclusion Antiox-LDL IgM subclass antibody 3A6 can effectively prevent the binding of unactivated macrophages to ox-LDL,reduce the occurrence and progression of atherosclerosis.