Serum/glucocorticoid regulated kinase 1 promotes adventitial fibroblasts phenotypic transformation
10.3969/j.issn.1009-0126.2018.03.019
- VernacularTitle:血清和糖皮质激素诱导的蛋白激酶1介导外膜成纤维细胞表型转化
- Author:
Yanan DU
1
;
Jianzhong XU
;
Baoli ZHANG
;
Weiqing HAN
;
Pingjin GAO
;
Xiaofeng TANG
Author Information
1. 200025,上海交通大学医学院附属瑞金医院高血压科
- Keywords:
glucocorticoids;
protein kinases;
transforming growth factor beta 1;
collagen type Ⅰ
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2018;20(3):294-297
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether SGK1 is involved in the phenotypic transformation in adventitial fibroblasts (AF).Methods Vascular AF were separated from the thoracic aortas of SD rats.The AF not stimulated with TGF-β1 were divided into blank group,control group 1 and control group 2.The AF induced with 2.5,5.0,10.0 and 15.0 ng/ml TGF-β1 were divided into groups A-D.The AF pretreated with 50 μmol/1 EMD638683 and SB203580 were divided into inhibitor group 1 and inhibitor group 2.The AF stimulated with 5 ng/ml TGF-β1 were divided into stimulation group 1 and stimulation group 2.The expression of SGK1,α-SMA and collagen Ⅰ was detected by Western blot.Results The expression of SGK1 was significantly higher in groups AC than in blank group.The expression of α-SMA was significantly higher in groups B-D than in controlgroup (P＜0.05).The α-SMA and collage Ⅰ expression levels were significantly higher in stimulation group 1 than in control group 1,and were significantly lower in inhibitor group 1 than in stimulation group 1 (P＜0.05).The SGK1 expression level was significantly higher in stimulation group 2 than in control group 2 and was significantly lower in inhibitor group 2 than in stimulation group 2 (P＜0.05).Conclusion SGK1 participates in TGF-β1-induced phenotypic transformation via p38 MAPK and is thus involved in vascular remodeling.
