Immunohistochemical Evaluation of Cathepsin D, MMP-2, and TIMP in Prostate Carcinoma.
- Author:
Jung Weon SHIM
;
Soon Ran KIM
;
Yun Jung KIM
;
Hye Kyung AHN
;
Young Euy PARK
;
Sung Sook KIM
;
Min Young KIM
- Publication Type:Original Article
- Keywords:
Prostate carcinoma;
Cathepsin D;
MMP-2;
TIMP
- MeSH:
Adenocarcinoma;
Animals;
Cathepsin D*;
Cathepsins*;
Cytoplasm;
Mice;
Prostate*
- From:Korean Journal of Pathology
1997;31(4):342-350
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Twenty six cases of primary adenocarcinoma of the prostate, ranging from 4 to 9 according to Gleason's summing score, were studied. Immunoreactivity was evaluated using the rabbit polyclonal anti-Cathepsin D antibody (CD), a mouse monoclonal MMP-2 antibody (MMP-2), and a tissue inhibitor metalloproteinase (TIMP) in formalin-fixed, paraffin-embedded prostatic tissue. Immunohistochemical staining was scored by summing the intensity of staining (0 to 3+) weighted by the percentage of tumor staining at each intensity (H score, theoretical range 0 to 300). For CD, the tumor cells showed diffuse cytoplasmic immunoreactivity in all 26 cases (100%). For MMP-2 the tumor cells showed cytoplasmic immunoreactivity in 17 of 26 cases (65.38%). As the Gleason grade increased the expression of CD increased (P=0.0027). The reactivity of CD was significantly correlated with the Gleason's score (R=0.65637), but, the reactivity of MMP-2 was not correlated. There were no significant correlations between each of the CD and the MMP-2 scores, and stage. TIMP expression was predominantly localized in the stroma rather than in the cancer cells themselves. We believe that 1) CD and MMP-2, both immunohistochemically detectable in a majority of prostate adenocarcinoma, may play a role in determination of the invasive or metastatic property, 2) the enhanced TIMP expression in the stroma may be associated with the response to cancer invasion.
