Relationship of TN-C, MMP-9 and TGF-β1 with aorta atherosclerotic plaues in mice
10.3969/j.issn.1009-0126.2018.02.021
- VernacularTitle:肌腱蛋白C和基质金属蛋白酶9及转化生长因子β1与小鼠主动脉斑块的关系
- Author:
Junwen HUANG
1
;
Yan LI
;
Jiacheng SONG
;
Zhanlong MA
;
Haibin SHI
Author Information
1. 210029,南京医科大学第一附属医院放射科
- Keywords:
apolipoproteins E;
matrix metalloproteinase 9;
transforming growth factor beta 1;
mice,knockout;
atherosclerosis
- From:Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2018;20(2):191-195
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship of TN-C,MMP-9 and TGF-β1 expression with aorta atherosclerotic plaue stability in mice on long-term high fat diet.Methods Fifty male apo E/ mice on high fat diet served as an experimental group and 50 male C57BL/6 mice on basic diet served as a control group.The morphology of plaques was observed with HE staining and the expression of TN-C,MMP-9 and TGF-β1 was detected with immunohistochemical staining.Results The serum TC and LDL-C levels were significantly higher in experimental group than in control group at weeks 16,24,32 and 40 (P＜0.05).The serum TG level was significantly higher in experimental group than in control group at week 16 (P＜0.05) and was significantly lower in experimental group than in control group at week 40 (P＜0.05).With the lengthening of the feeding time,the plaque area,the ratio of plaque to lumen area,and the expression of TN-C and MMP-9 increased gradually,but the expression of TGF-β1 decreased gradually (P＜0.05).Conclusion The expression of TN-C,MMP-9 and TGF-β1 can show the stability of atherosclerotic plaques.
