Effect of pioglitazone on expression of p38 mitogen-activated protein kinase in myocardium of rats following I/R injury
10.3969/j.issn.1009-0126.2018.02.020
- VernacularTitle:吡格列酮对大鼠缺血再灌注心肌p38丝裂原活化蛋白激酶表达的影响
- Author:
Hao WANG
1
;
Ping YE
;
Qiwei ZHU
;
Leiming LUO
Author Information
1. 解放军总医院南楼心血管内科国家老年疾病临床医学研究中心
- Keywords:
reperfusion injury;
p38 mitogen-activated protein kinases;
PPAR gamma;
apoptosis
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2018;20(2):188-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of pioglitazone,a member of the thiazolidinedione (TZD) class with hypoglycemic action to treat diabetes,on expression of p38 mitogen-activated protein kinase in myocardium of rats following I/R injury.Methods Twenty-four healthy SD rats were randomly divided into sham group,I/R injury group,pioglitazone treatment group and pioglitazone+peroxisome proliferator-activated receptors-γ specific antagonist GW9662 treatment group (6 in each group).A rat I/R injury model was established by ligating the left anterior descending coronary artery.Apoptosis of myocardial cells was assayed by Terminal dUTP deoxynucleotidyl transferase nick end labeling.Expression of p-p38 protein was detected by Western blot.Results The apoptosis index of myocardial cells was significantly lower in sham operation group and piogl itazone treatment group than in I/R injury group and was significantly higher in pioglitazone+ GW9662 treatment group than in pioglitazone treatment group (8.6％±4.3％,21.4％±8.8％ vs 40.1％±12.3％,P＜0.05;37.0％-10.5％ vs 21.4％±8.8％,P＜0.05).The expression level of p-p38 was significantly lower in sham operation group and pioglitazone treatment group than in I/R injury group and was significantly higher in pioglitazone+GW9662 treatment group than in pioglitazone treatment group (P＜0.05).Conclusion Pioglitazone can inhibit I/R injury-induced apoptosis of myocardial cells by downregulating the expression of p-p38 protein.
