Mechanism of cucurbitacin B underlying pressure overload-induced myocardial fibrosis
10.3969/j.issn.1009-0126.2018.02.019
- VernacularTitle:葫芦素B对压力负荷诱导的心肌纤维化的作用机制
- Author:
Mingxia DUAN
1
;
Qingqing WU
;
Qizhu TANG
Author Information
1. 430060,武汉大学人民医院心血管内科武汉大学心血管病研究所心血管病湖北省重点实验室
- Keywords:
cucurbitacins;
endomyocardial fibrosis;
actins;
transforming growth factor beta;
ligation
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2018;20(2):184-187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of cucurbitacin B (CuB) underlying pressure over load-induced myocardial fibrosis.Methods Sixty C57 mice were divided into sham operation group,CuB treatment group,aorta ligation group,CuB+aorta ligation group (15 in each group).The animals received intragastric gavage (0.2 mg/kg · 2 d) one week after operation and a myocardial fibrosis model of mice was induced by aorta ligation 4 weeks after operation.Microvascular density (MVD) was detected with immunohistochemical staining.Expressions of α-SMA,CD31,CD34,vimentin and endothelial-mesenchymal transition (EndMT) were detected by Western blot with immunofluorescence staining.Results No significant difference was found in cardiac MVD,and expression level of α-SMA,vimentin,CD31 and CD34 between sham operation group and CuB group 4 weeks after operation (P＞0.05).The cardiac MVD and expression level of CD31 and CD34 were significantly lower while the expression level of α-SMA and vimentin was significantly higher in aorta ligation group than in sham operation group 4 weeks after operation (P＜0.05).The cardiac MVD and expression level of CD31 and CD34 were significantly higher while the expression level of α-SMA and vimentin was significantly lower in CuB+aorta ligation group than in aorta ligation group 4 weeks after operation (P＜0.05).Conclusion CuB can attenuate cardiac fibrosis by regulating EndMT.
