Expression of BDNF and TrkB in hippocampal microglia cells of poststroke depression rats
10.3969/j.issn.1009-0126.2018.01.021
- VernacularTitle:脑卒中后抑郁大鼠海马小胶质细胞脑源性神经营养因子及酪氨酸激酶受体B蛋白的表达
- Author:
Jia SHI
1
;
Dan DAI
;
Yangyang LI
;
Min CHEN
;
Wanli FU
;
Yun LI
Author Information
1. 671000,大理大学临床医学院
- Keywords:
stroke;
depression;
microglia;
hippocampus;
brain-derived neurotrophic factor;
receptor protein-tyrosine kinases
- From:Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2018;20(1):83-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of BDNF and TrkB in hippocampal microglia cells of poststroke depression (PSD) rats.Methods Forty healthy adult SD rats were randomly divided into normal group,depression group,stroke group and PSD group (10 in each group).Expression of BDNF and TrkB in OX42-marked hippocampal microglia cells of PSD rats was detected with immunofluorescence staining on day 29 and 57 after a MCAO model rats was established and a chronic stress depression model of rats was established by chronic unpredictable mild stress (CUMS) combined with separate breeding.Results The absorbance value of BDNF and TrkB was 83.35t5.74 and 82.35±5.74 respectively in PSD group on day 29 after the CUMS of rats was established,which was significantly lower than other 3 groups (P＜0.05),and was significantly higher in depression group than in stroke group (141.23±9.16 vs 133.31±7.89;141.23± 8.07 vs 128.62±6.92,P＜0.05).The absorbance value of BDNF and TrkB was 81.63±7.19 and 74.43±7.42 respectively in PSD group on day 57 after the CUMS of rats was established,which was lower than in other 3 groups,and was significantly lower in stroke group than normal group and depression group (93.36±7.56 vs 124.11±11.39、116.65±10.55,87.14±6.56 vs 112.58± 10.99、108.05±10.57,P＜0.05).Conclusion Hippocampal microglia cells play an important role in the pathogenesis of PSD by down-regulating the expression of BDNF and TrkB in PSD rats.
