Establishment of a time-resolved fluoroimmunoassay in detection of human epidermal growth factor receptor 2
10.3760/cma.j.issn.2095-2848.2018.05.007
- VernacularTitle:人表皮生长因子受体2时间分辨荧光免疫分析法的建立
- Author:
Jun FAN
1
;
Yi ZHANG
;
Ke WANG
;
Wenwei SUN
;
Jue ZHANG
;
Bin ZHOU
;
Lixin FU
;
Biao HUANG
;
Qin YANG
Author Information
1. 江苏省原子医学研究所、卫生部核医学重点实验室、江苏省分子核医学重点实验室
- Keywords:
Receptor,epidermal growth factor;
Breast neoplasms;
Fluoroimmunoassay
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2018;38(5):336-339
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a time-resolved fluoroimmunoassay (TRFIA) method for human epidermal growth factor receptor 2 (HER2) detection and to evaluate its performance.Methods Each well of the 96-microwell plate was coated with monoclonal antibody of HER2(H7) and another monoclonal antibody of HER2(E5) was labeled by Eu3+.The sensitivity,stability,specificity,measurement range and reference value of this method were tested.The correlation between chemiluminescence (CLIA) method and TRFIA method was analyzed.Results The sensitivity of HER2-TRFIA method was 0.214 ng/ml.The measurement range was 0.214-1 000 ng/ml.The mean within-run CV and mean between-run CV were 3.48％ and 4.13％,respectively.HER2-TRFIA method had no cross-reaction with HER1 and its reference range was 0-13.20 ng/ml.The correlation coefficient between TRFIA and CLIA was 0.997.The same batch of reagents were found to be stable for more than 6 months at 4 ℃.Conclusions HER2-TRFIA method has high sensitivity,specificity,stability and wide detecting range.It might be suitable for clinical use.
