Protease activated receptor-1 targeted 99Tcm-(HYNIC-BMS-200261) (tricine) (TPPTS) imaging on nude mice bearing human breast cancer
10.3760/cma.j.issn.2095-2848.2018.03.011
- VernacularTitle:以蛋白酶激活受体-1为靶点的99Tcm-(HYNIC-BMS-200261)(tricine) (TPPTS)荷乳腺癌裸鼠显像
- Author:
Jie LIU
1
;
Xiaojian LIU
;
Honglei LI
;
Chaoling JIN
;
Meng WANG
;
Wei FANG
;
Haojie DAI
;
Jue YAN
;
Yumin ZHENG
Author Information
1. 中日友好医院核医学科
- Keywords:
Breast neoplasms;
Receptors,proteinase-activated;
Antistatic agents;
Radionuclide imaging;
Tumor cells,cultured;
Mice,nude
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2018;38(3):195-198
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the reliability of 99Tcm-(hydrazinonicotinamide (HYNIC)-BMS-200261) (tricine) (trisodium triphenylphosphine-3,3',3"-trisulfonate (TPPTS)) as a radiotracer for protease activated receptor-1 (PAR-1) expression in breast cancer.Methods Fifteen nude mice bearing MDA-MB-435,MDA-MB-231 and MCF-7 human breast cancer xenografts (5 mice for each cell line) with different PAR-1 expression were used for 99Tcm-(HYNIC-BMS-200261) (tricine) (TPPTS) γ imaging,and tumor/non-tumor (T/NT) ratios were obtained with region of interest (ROI) technique.Afterwards,the biodistribution of 99Tcm-(HYNIC-BMS-200261) (tricine) (TPPTS) was analyzed in tumor-bearing nude mice,and the radioactivity in tumor (percentage activity of injection dose per gram of tissue,％ID/g) was calculated.The immunostaining was performed to examine PAR-1 expression in tumor tissue and semi-quantitative analysis was used.One-way analysis of variance and Pearson correlation analysis were used to analyze data.Results At 2 h postinjection,the T/NT ratios were 3.03±0.32,2.27±0.25 and 1.51±0.13 respectively in nude mice bearing MDA-MB-435,MDA-MB-231 and MCF-7 xenografts;the tumor uptakes of 99Tcm-(HYNIC-BMS-200261)(tricine)(TPPTS) were (1.03±0.15),(0.56±0.14) and (0.30±0.06) ％ID/g;PAR-1 expression levels were (17.22±2.71) ％,(10.78± 1.95) ％ and (2.80± 1.18) ％,respectively (F values:47.66,46.36,62.35,all P＜0.05).The T/NT ratios and tumor uptake of 99Tcm-(HYNIC-BMS-200261) (tricine) (TPPTS) at 2 h post-injection were correlated with PAR-1 expression (r values:0.934 and 0.929,both P＜0.05).Conclusions 99Tcm-(HYNIC-BMS-200261) (tricine) (TPPTS) imaging could be a noninvasive and effective method for monitoring PAR-1 expression in human breast cancer.
