Function of mucin 2, mucin 4 on lithogenic bile of calculus of intrahepatic duct
10.3760/cma.j.issn.1007-8118.2018.09.009
- VernacularTitle:黏蛋白2与黏蛋白4在肝内胆管结石致石胆汁形成中的作用
- Author:
Jinhai LI
1
;
Minghui ZHU
;
Yu CAI
;
Haifeng ZHANG
;
Shenghua PAN
;
Qingfan PU
Author Information
1. 温州医科大学附属第三医院肝胆外科
- Keywords:
Calculus of intrahepatic duct;
Lithogenic bile of gallstone;
Mucin 2;
Mucin 4;
Cholecithiasis
- From:
Chinese Journal of Hepatobiliary Surgery
2018;24(9):609-612
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function of mucin 2,mucin 4 on formation of lithogenic bile in patients with calculus of intrahepatic duct.Methods Bile duct mucosa,bile duct wall,bile and plasma were collected from 56 patients with calculus of intrahepatic duct (CID group) and 17 individuals without calculus of intrahepatic duct (control group).The bile duct wall was stained with mucin 2 (MUC2) and mucin 4 (MUC4).Reverse-transcription polymerase chain reaction (RT-PCR) was applied to study the mRNA expressions of MUC2 and MUC4 in the bile duct mucosa.The correlation of the bile duct and serum lipid index was analyzed.Results Serum lipid index in the CID group was significantly higher than control group (all P＜0.05).Biliary total bile acids and bile acids mol percentage were lower in the CID group than control group (both P＜0.05).The expressions of MUC2 was not increased significantly in CID group than the control group (all P＞0.05).The expressions of MUC4 were more significantly increased in CID group than the control group (P＜0.05).The mRNA of MUC4 in the CID group was also more significantly increased than in control group (P＜0.01).There were no correlations between MUC4 expression and the level of biliary total bile acid in the CID group (r=-0.374,P＜0.05).Conclusion The expression of MUC4 in patients with CID was enhanced,which promoted the absorption of bile acid by the mucosal epithelium of the bile duct,and caused a large amount of mucin to be secreted into bile,which may be related to the formation of stony bile.
