Relationships between peripheral circulating tumor cells in patients with gallbladder carcinoma detected by a novel nano microfluidic chip with clinicopathology and prognosis
10.3760/cma.j.issn.1007-8118.2018.05.009
- VernacularTitle:新型纳米微流控芯片检测胆囊癌患者外周血CTCs与临床病理及预后的关系
- Author:
Xiaoguang WANG
1
;
Bin WU
;
Quanfa NI
;
Lifeng QI
;
Jianguo FEI
;
Zhengxiang ZHONG
Author Information
1. 314000,嘉兴医学院附属第二医院肝胆外科
- Keywords:
Gallbladder carcinoma;
Microfluidic chip,nano;
Circulating tumor cells (CTCs);
Prognosis
- From:
Chinese Journal of Hepatobiliary Surgery
2018;24(5):321-324
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a novel method using a nano microfluidic chip to detect circulating tumor cells (CTCs) in peripheral blood in gallbladder carcinoma,and to study the relationship between CTCs with clinicopathology and prognosis in these patients.Methods The peripheral blood samples of 51 patients with gallbladder carcinoma were collected from June 2014 to January 2017.The CTCs from peripheral blood samples were detected by a novel nano microfluidic chip.This study aimed to study the correlation between CTCs with the clinical and pathological features.The significance of CTCs on prognosis in patients with gallbladder carcinoma was also analyzed.Results The positive rate of CTCs in the peripheral blood of gallbladder carcinoma patients was 43.1％ (22/51).There were significant correlations between CTCs with liver metastasis (P ＜ 0.05) and Nevin staging (P ＜ 0.05).The 1-and 2-year overall survival (OS) in patients with CTCs were 70.7％ and 35.3％,and the 1-and 2-year OS in patients without CTCs were 92.0％ and 56.1％.There was a significant difference in the 2-year OS (P ＜ 0.05) but no significant difference in the 1-year OS between the 2 groups of patients (P ＞ 0.05).Conclusions Peripheral blood CTCs in patients with gallbladder carcinoma were efficaciously detected by a novel nano microfluidic chip.Peripheral blood CTCs was closely related to the Nevin staging and liver metastasis.CTCs could serve as a potential prognostic indicator in patients with gallbladder carcinoma.
