The effects of targeted inhibition of hedgehog signaling pathway on proliferation, apoptosis and metastasis of hepatocellular carcinoma
10.3760/cma.j.issn.1007-8118.2018.03.007
- VernacularTitle:靶向抑制Hedgehog信号通路对肝细胞癌增殖、凋亡和转移的影响
- Author:
Yimeng SHEN
1
;
Qingxiang GAO
;
Xiaozhi LIU
;
Qiaohao WAN
;
Xiaodong TIAN
;
Yinmo YANG
;
Yanxia LI
;
Wenhan WU
Author Information
1. 100034,北京大学第一医院外科
- Keywords:
Hepatocelluar Carcinoma;
Signal transduction;
Hedgehog signaling pathway
- From:
Chinese Journal of Hepatobiliary Surgery
2018;24(3):167-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe Hedgehog signaling pathways of liver cancer cell growth and the influence of the metastatic potential targeted inhibit Hedgehog.Methods Construction of Smo shRNA plasmid,The stable and low-expressed Smo-expressing HCC QGY-7701 cell line was screened after lipofection.The stable and low-expressed Smo-expressing HCC QGY-7701 cell line was screened,The cell cycle,apoptosis,invasion and metastasis of QGY-7701 cells were detected by Western blot,flow cytometry,CCK8 and transwell assay.Subcutaneous implantation of hepatocarcinoma cells in nude mice.Study on the growth and metastasis of hepatocarcinoma cells with low expression of Smo in.The ultrastructural changes of hepatoma cells with low expression of Smo were observed under electron microscope.Results RT-PCR and Western blot showed stable shR-Smo cell line was successfully constructed.Cell cycle test showed that compared with the control group,G0/G1 cells increased in shR-Smo,cells in S phase decreased;apoptosis,CCK8 and Transwell tests showed that Smo-gene silencing could significantly increase the apoptosis percentage of the hepatic cancer cells to (5.46％ ± 1.46％),proliferation activity decreasedand and the migration rates reduced to (7.82％ ±2.14)％;nude mice model showed that Smo-gene silencing could inhibit the growth of hepatocellular carcinoma cells in vivo,electron microscopy revealed that lysosomes increased significantly in Smo-gene silence cells.Conclusions Blocking Hh signaling pathways,liver cancer cells in vitro malignant degree of decline.Hedgehog in treating liver cancer have hidden meaning.
