Effects and mechanism of Lanthanum Citrate on proliferation and apoptosis of human hepatocellular carcinoma cell line SMMC-7721
10.3760/cma.j.issn.1007-8118.2018.01.012
- VernacularTitle:柠檬酸镧对肝癌SMCC-7721细胞增殖凋亡的影响及其机制
- Author:
Bin CHEN
1
;
Zhengkuan DAI
;
Bofei LI
;
Chunhua WANG
Author Information
1. 赣南医学院第一附属医院肝胆外科
- Keywords:
Hepatocellular carcinoma;
Signaling pathway,Hedgehog;
Lanthanum Citrate;
Cell proliferation;
Cell apoptosis
- From:
Chinese Journal of Hepatobiliary Surgery
2018;24(1):49-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and the possible mechanism of Lanthanum Citrate on the proliferation and apoptosis of human hepatocellular carcinoma cell line SMMC-7721 through the Hedgehog signaling pathway.Methods Hhsignaling pathway inhibitor KAAD-cyclopamine and Lanthanum Citrate in different concentrations were used to treat human hepatocellular carcinoma cell line SMMC-7721.Cell proliferation was detected by MTT method.The apoptosis rate was detected by Annexin V/PI flow cytometry.The expressions of the regulative genes,such as CyclinD1,p21,Caspase-3,Bcl-2,Gli1,Shh were detected by Western blot.The mRNA expressions of Gli1 and Shh were detected by q-PCR.Results In control group,SMMC-7721 cells were treated with 0.1 mmol/L Lanthanum Citrate group,and 15 μnol/L KAAD-cyclopamine for 48 h,and expression of CyclinD1 protein in each group was (1.41 ± 0.21),(0.71 ±0.08) and (0.53 ±0.11);p21 protein was (0.49 ±0.08),(0.97 ±0.09) and (1.55 ±0.13);Caspase-3 protein was (0.36 ± 0.05),(0.67 ± 0.10) and (0.89 ± 0.11);the relative expression of Bcl-2 protein in each group was (0.61 ± 0.10),(0.48 ± 0.05) and (0.24 ± 0.08).Gli1,Shh mRNA and Gli1,Shh protein expression were decreased.AnnexinV-FITC double staining showed Lanthanum Citrate group and KAAD-cyclopamine group had increased apoptosis rate of SMMC-7721 cells by flow cytometry.Conclusion Lanthanum Citrate inhibits the proliferation and promotes apoptosis in hepatocellular carcinoma SMMC-772 cells through suppressing hedgehog signaling pathway.
