Effect of Cadmium and Zinc on IL-6 Secretion in a Human Monocytic Cell Line, THP-1.
- Author:
Dong Hoon SHIN
1
;
Suk Kwon SUH
;
Seong IL SUH
Author Information
1. Department of Preventive Medicine and Public Health, School of Medicine, Keimyung University, Korea.
- Publication Type:Original Article
- MeSH:
Cadmium Chloride;
Cadmium*;
Cell Culture Techniques;
Cell Line*;
Edetic Acid;
Gene Expression;
Humans*;
Interleukin-16;
Interleukin-6*;
Monocytes;
RNA, Messenger;
Zinc*
- From:Korean Journal of Occupational and Environmental Medicine
1999;11(3):332-339
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: This study was performed to investigate effects of cadmium and zinc on IL-6 secretion using human monocyte in a culture system. METHODS: We have used frypan blue dye exclusion methods to examine the effect of cytotoxicity of CdCl2, ZnCl2 in THP-1 cells. IL-6 was measured by the ELIBA method in the cell culture supernatants and the expression of IL-6 mRNA was examined by reverse transcription-polymerase chain reaction. RESULTS: IL-6 production by THP-1 cells were decreased at 0.1 mM cadmium concentration(p < 0.05) and increased as zinc concentration increased. Zinc prevented cadmiuminduced suppression of IL-6. and the addition of a chelating agent, EDTA, restores IL-6 secretion. The expression of IL-6 mRNA decreased at 0.08 mM and 0.1 mM cadmium concentrations. CONCLUSION: This results suggests that cadmium depressed production and gene expression of IL-6 in stimulates human monocytes, and zinc prevented cadmium-induced suppression of IL-16.
