Titanium dioxide nanoparticles coupled with nuclear localization sequence increases the radiosensitivity of U251 glioma cells
10.3760/cma.j.issn.0254-5098.2018.11.001
- VernacularTitle:核定位序列修饰的二氧化钛纳米颗粒对胶质瘤U251细胞的放射增敏作用
- Author:
Jun SHANG
1
;
Ting XIE
;
Narui YIN
;
Fei CHEN
;
Jie DU
;
Haowen ZHANG
;
Jiahua YU
;
Fenju LIU
Author Information
1. 215123,苏州大学医学部放射医学与防护学院 放射医学与辐射防护国家重点实验室江苏省放射医学协同创新中心 江苏省放射医学与防护重点实验室
- Keywords:
Titanium dioxide nanoparticles;
Nuclear localization sequence;
Radiosensitivity;
Glioma cells
- From:
Chinese Journal of Radiological Medicine and Protection
2018;38(11):801-806
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of titanium dioxide ( TiO2 ) nanoparticles coupled with nuclear localization sequence ( NLS ) on the radiosensitivity of U251 glioma cells. Methods Synthesis and characterization of the TiO2-NLS nanoparticles with nuclear targeting property. U251 cells were treated with nanoparticles and/or ionizing radiation. Flow cytometry analysis was performed to measure the ROS content and the cell apoptotic percentage. The DNA damage was detected by using γ-H2AX foci staining. Clonogenic survival assay was used to evaluate the radiosensitivity of U251 cells. Results NLS modification promoted nuclear translocation of TiO2 nanoparticles. Compared with the control nanoparticles, TiO2-NLS treatment increased radiation-induced cell apoptosis (t=8. 96, P<0. 05). Clonogenic survival assay showed the radiosensitization ratios of TiO2 nanoparticle-treated group and TiO2-NLSnanoparticle-treated group were 1. 18 and was 1. 29, respectively. These two ratios had statistically difference ( t =14. 72, P< 0. 05). Conclusions Nuclear targeting TiO2 nanoparticles enhances the radiosensitivity of U251 glioma cells.
