Role of connexin 43 in X-ray induced apoptosis in human umbilical vein endothelial cells and its mechanism
10.3760/cma.j.issn.0254-5098.2018.10.002
- VernacularTitle:缝隙连接蛋白43在X射线诱导的人脐静脉内皮细胞凋亡中的作用及机制研究
- Author:
Chen LI
1
;
Mei TIAN
;
Qiao GOU
;
Jianxiang LIU
;
Xuesong QI
;
Chunyan WANG
;
Xu SU
Author Information
1. 中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室
- Keywords:
X-rays;
HUVEC cell;
Cx43;
Apoptosis;
cleaved caspase-3
- From:
Chinese Journal of Radiological Medicine and Protection
2018;38(10):728-733
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of Cx43 in X-ray induced apoptosis of HUVEC cells and its mechanism.Methods Flow cytometry was used to detect the apoptosis of HUVEC cells at 48-96 h after 10 Gy X-ray irradiation and at 72 h after irradiation of different doses.Western blot was used to detect the protein expressions of Cx43 and cleaved caspase-3 in HUVEC cells at 72 h after 0,5,10 and 20 Gy irradiation.Small interfering RNA was transfected into HUVEC cells to silence Cx43 expression,the Cx43 bearing plasmid was transfected into cells to overexpress Cx43.The effect of Cx43 knockdown or overexpression on apoptosis induction and cleaved caspase-3 protein expression were detected by flow cytometry and Western blot,respectively.Results The apoptosis of HUVEC increased significantly from 48 h to 96 h after X-ray irradiation and in a dose-dependent manner at 72 h after irradiation.The expression of Cx43 protein was negatively correlated with the dose but the expression of cleaved caspase-3 was positively correlated with the dose in the range of 0-20 Gy.After Cx43 silencing,the proportion of early apoptosis and apoptosis combined with dead cells were significantly higher than that of the siRNA control group(t =3.674,6.375,P ＜ 0.05).After Cx43 overexpression,the proportion of early apoptosis and apoptosis combined with dead cells were significantly lower than that of vector control group(t =9.399,11.190,P ＜ 0.05).The expression of cleaved caspase-3 in the Cx43 silencing group was higher than that in the siRNA control group,but this protein in the Cx43 overexpressed group was lower than that in the vector control group.Conclusions Cx43 may protect X-ray irradiated HUVEC cells from apoptosis by down-regulating the activation of caspase-3.
