Establishment of an intestinal organoid-based method to investigate the radiation mitigation effect of Epiregulin
10.3760/cma.j.issn.0254-5098.2018.09.001
- VernacularTitle:利用肠道类器官培养研究表皮调节素的抗辐射作用
- Author:
Guoxiang FU
1
;
Yuanchuang LI
;
Mengxue PAN
;
Qiang GUO
;
Xinxin RAO
;
Xiaoya XU
;
Yi ZHOU
;
Chunlin SHAO
;
Jianjun GAO
;
Guoqiang HUA
Author Information
1. 复旦大学放射医学研究所
- Keywords:
Intestinal stem cell;
Intestinal Organoid;
Radiation;
Epiregulin;
Radiation-induced gastrointestinal syndrome
- From:
Chinese Journal of Radiological Medicine and Protection
2018;38(9):641-646
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an intestinal organoid-based assay to investigate the radiation mitigation effect of epiregulin in vitro. Methods Intestinal crypts were released from tissue incubated with EDTA. Intestinal crypts seeded in 3D matrigel were irradiated at 24 h after plating. The radiation mitigation effect of epiregulin was evaluated by measuring the survival rate, size and budding numbers of the organoid after irradiation, and the basic FGF was used as a positive control of epiregulin. Results Radiation-induced lethality and dose-dependent survival curve of the intestinal organoid were consistent with in vivo data. Treatment with epiregulin (400 ng/ml) at 24 h post-radiation significantly increased survival rate of 8 Gy X-ray irradiated intestinal organoid in comparison with non-treated group [(12.56 ± 1.02)％vs. (4.73 ± 0.38)％, t=12.43,P<0.05]. Conclusions Epiregulin has radiation mitigation effect on intestinal organoid and could serve as a potential medical countermeasure to mitigate gastrointestinal toxicity.
