The protective effect of hydrogen-rich water on irradiation-induced cognitive dysfunction
10.3760/cma.j.issn.0254-5098.2018.06.003
- VernacularTitle:富H2水对电离辐射所致认知功能损伤的保护作用
- Author:
Mengya LIU
1
;
Jianbo SONG
;
Jie GAO
;
Hui YUAN
;
Yongli WANG
;
Xingang WANG
;
Juan YUE
;
Xiujun QIN
;
Jianguo LI
;
Jianhua WEN
Author Information
1. 中国辐射防护研究院 药物毒理与放射损伤药物山西省重点实验室
- Keywords:
Hydrogen-rich water;
Ionizing radiation;
Cognitive dysfunction;
Radiation protection
- From:
Chinese Journal of Radiological Medicine and Protection
2018;38(6):414-418,465
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of hydrogen-rich water on rat cognitive dysfunction induced by ionizing radiation.Methods A total of 20 SD rats were randomly divided into four groups with the ramdom number table method: control group(C),hydrogen-rich water group(HRW),irradiation group(IR)and hydrogen-rich water intervention group(HRW+IR),with 5 rats in each group.The spatial memory ability of rats was tested by a morris water maze.The expression of apoptosis-related genes was detected by real-time fluorescence quantitative PCR.The changes of glutathione(GSH),8-hydroxydeoxy guanosine(8-OHdG)and malondialdehyde(MDA)and SOD were also measured.Results The escape latency(F＝6.003,P＜0.05)and the swimming distances(F＝3.850,P＜0.05)of rats in four different groups had statistically significant differences.Compared with the IR group,the escape latency of the HRW+IR group was significantly decreased at 3,4,5 d after irradiation(P＜0.05),and the swimming distance of this group became much longer(P＜0.05).The levels of GSH,8-OHdG and MDA in these four groups had statistically significant differences(F＝6.450,5.033,4.113,P＜0.05).Compared with IR group,the concentration of GSH was increased(P＜0.05),but MDA and 8-OHdG decreased(P＜0.05)in the brain tissue of HRW+IR group,and the expressions of caspase-3,caspase-9 and bax genes were reduced(t＝2.956,3.087,5.246,P＜0.05),while the expression of bcl-2 gene was enhanced(t ＝-3.640,P ＜0.05)in the HRW+IR group.Conclusions Hydrogen-rich water attenuates the oxidative damage of ionizing radiation by neutralizing oxyhydrogen free radicals and thus protects brain from radiation damage.
