The dynamic changes of gene expressions in radiation-injured intestine based on RNA-sequence analysis
10.3760/cma.j.issn.0254-5098.2018.02.001
- VernacularTitle:基于转录组测序的放射性肠损伤基因动态变化
- Author:
Jinhan WANG
1
;
Qianying LU
;
Kaihua JI
;
Liqing DU
;
Yan WANG
;
Chang XU
;
Qiang LIU
Author Information
1. 中国医学科学院北京协和医学院放射医学研究所天津市放射医学与分子核医学重点实验室
- Keywords:
RNA-seq;
Radiation-induced intestinal injury;
Differential expression genes;
Signaling pathways;
Gene interaction network
- From:
Chinese Journal of Radiological Medicine and Protection
2018;38(2):81-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic changes of gene expressions in mouse jejunum after lethal dose abdomen irradiation (ABI).Methods RNA was extracted from mouse jejunum at 0 and 6 h,3.5 and 5 d after 14 Gy 137Cs γ-ray ABI and then subjected to RNA-sequence analysis.Gene with expressions changed more than 2-fold of control were identified as differentially expressed ones.The selected genes were subsequently analyzed using IPA,Funrich,GO and KEGG software.Results Gene analysis of mouse jejunum samples showed that radiation activated p53 pathway at 6 h and 3.5 d after ABI.Interaction network analysis of genes suggested that Lck,Cdkl and Fyn,genes could play an important role in jejunum damage at 3.5 d after ABI.The gene expression profiles demonstrated that ABI up-regulated DNA damage repair pathways and down-regulated cell adhesion molecules,focal adhesion and IgA production pathways.Conclusions The p53 signaling pathway and some key genes such as Lck,Cdkl,and Fyn may contribute to the radiation-induced intestinal injury.
