Radiosensitization of irisquinone on the Warburg effect of MDA-MB231 cells
10.3760/cma.j.issn.0254-5098.2018.01.001
- VernacularTitle:马蔺子素放射增敏作用对MDA-MB231细胞Warburg效应的影响
- Author:
Xiaoyu SU
1
;
Huiqin XU
;
Hui WANG
;
Dan ZHANG
;
Feng QIAO
;
Wenjing YU
Author Information
1. 安徽医科大学第一附属医院核医学科
- Keywords:
Irisquinone;
Radiosensitization;
Warburg effect;
HK Ⅱ
- From:
Chinese Journal of Radiological Medicine and Protection
2018;38(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the radiosensitization of irisquinone on the Warburg effect of MDA-MB231 cells.Methods MDA-MB231 cells in the exponential growth phase were divided into 6 groups:control group,irisquinone group,radiation group,irisquinone plus radiation group(Irisquinone + RA),negative control group,and experimental group (siRNA).Colony formation assay was used to measure cell survival fraction of MDA-MB231 cells.Single-hit multi-target model was used to fit the survival curve and calculate the sensitive enhancement ratio (SER).Flow cytometry was used to measure cell apoptosis.The relative expression levels of HK Ⅱ mRNA and protein in each group were detected by qRT-PCR and Western blot respectively.Results The values of D0,Dq and SF2 in the irisquinone plus radiation group were obviously lower than those in the radiation group.The SER of irisquinone was 1.52.Compared with the other groups,the cell apoptosis rate was increased (t =13.29,12.09,5.90,3.83,P ＜ 0.05),while the relative expression levels of HK Ⅱ mRNA (t =9.14,10.48,3.40,P＜0.05) and protein (t=13.39,16.08,5.81,P ＜ 0.05) were decreased in the irisquinone plus radiation group significantly.Conclusions Radiosensitization function of irisquinone inhibits the Warburg effect of MDA-MB231 cells by down-regulating the expression of HK Ⅱ.
