Changes of estrogen and its receptor ERα, ERβ and GPR30 in asymptomatic hyperuricemia
10.3760/cma.j.issn.1007-7480.2018.08.007
- VernacularTitle:雌激素及雌激素受体α雌激素受体βG蛋白偶联受体在男性无症状高尿酸血症中的变化
- Author:
Menglan LI
1
;
Wantai DANG
;
Xiaoshuang YIN
;
Xinyi HE
;
Hongbing SUN
;
Tianhong LI
;
Jing YANG
;
Jingguo ZHOU
;
Shiquan SHUAI
Author Information
1. 370006,四川省南充市中心医院川北医学院第二临床医学院风湿免疫科
- Keywords:
Estrogen;
Receptor,estrogen;
Hyperuricemia
- From:
Chinese Journal of Rheumatology
2018;22(8):537-542
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes aad possible role of estrogen and its receptor ERα、ERβ、GPR30 in the pathogenesis of asymptomatic hyperuricemia.Methods The peripheral blood of 62 asymptomatic hyperuricemia patients (AH) and 68 healthy controls (HC) were collected.The expression of estradial (E2) in serum was detected by the chemilluminescent microparticle immunoassay (CMIA).The expression of ERα,ERβ,GPR30 mRNA in peripheral blood mononuclear cells (PBMCs) was measured using Real time quantitative polymerase chain reaction (RT-qPCR).Statistical Package form Soci-science (SPSS) 17.0 statistical software was used for data analysis.The measurement data were compared by t test,rank sum test or one factor analysis of variance test.The correlation between variables was used by Spearman correlation analysis.Results ① The expression of E2 in serum of the HC group was higher than that in the AH group [(38.7±10.2) pg/ml vs (33.7±8.6) pg/ml,Z=-0.356,P＜0.05].② The expression of ERα,GPR30 mRNA in PBMCs of HC group was increased,compared with that in the AH group (0.000 17±0.000 23 vs 0.000 12± 0.000 12,0.002 0±0.002 1 vs 0.001 5±0.000 8,Z=-2.112,-2.147,P＜0.05,respectively).No significant difference in PBMCs ERβ mRNA levels was found between HC group and AH group,while a slight but not significant increase was observed in HC group.③ The Spearman correlation analysis found that the expression of ERα and ERβ mRNA,E2 and GR,ERβ and GLU in the AH group were positively related (r=0.259,0.251,0.260,P＜0.05,respectively).Conclusion The expression of E2,ERα,ERβ,GPR30 mRNA in the peripheral blood of patients with AH is decreased,suggesting that the estrogen and its receptor may be involved in the patho-genesis of hyperuricemia.
