Role of BoBs technology in early missed abortion chorionic villi
10.3760/cma.j.issn.0529-567x.2018.05.005
- VernacularTitle:BoBs分子诊断技术在早期自然流产遗传学分析中的应用及评价
- Author:
Zhiyi LI
1
;
Xinyan LIU
;
Ping PENG
;
Na CHEN
;
Jie OU
;
Na HAO
;
Jing ZHOU
;
Xuming BIAN
Author Information
1. 100730,中国医学科学院北京协和医学院北京协和医院妇产科
- Keywords:
Abortion;
missed;
Molecular diagnostic techniques;
Chromosomes;
artificial;
bacterial;
Karyotyping
- From:
Chinese Journal of Obstetrics and Gynecology
2018;53(5):308-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the value of bacterial artificial chromosome-on-beads(BoBs) technology in the genetic analysis of early missed abortion chorionic villi. Methods Early missed abortion chorionic villi were detected with both conventional karyotyping method and BoBs technology in Peking Union Medical Hospital from July 2014 to March 2015. Compared the results of BoBs with conventional karyotyping analysis to evaluate the sensitivity, specificity and accuracy of this new method. Results (1) A total of 161 samples were tested successfully in the technology of BoBs, 131 samples were tested successfully in the method of conventional karyotyping.(2)All of the cases obtained from BoBs results in(2.7 ± 0.6) days and obtained from conventional karyotyping results in (22.5 ± 1.9) days. There was significant statistical difference between the two groups(t=123.315, P<0.01).(3)Out of 161 cases tested in BoBs, 85(52.8%, 85/161)cases had the abnormal chromosomes, including 79 cases chromosome number abnormality, 4 cases were chromosome segment deletion, 2 cases mosaic. Out of 131 cases tested successfully in conventional karyotyping, 79(60.3%, 79/131)cases had the abnormal chromosomes including 62 cases chromosome number abnormality, 17 cases other chromosome number abnormality, and the rate of chromosome abnormality between two methods was no significant differences(P=0.198).(4)Conventional karyotyping results were served as the gold standard, the accuracy of BoBs for abnormal chromosomes was 82.4%(108/131), analysed the normal chromosomes (52 cases) and chromosome number abnormality (62 cases)tested in conventional karyotyping, the accuracy of BoBs for chromosome number abnormality was 94.7%(108/114). Conclusion BoBs is a rapid reliable and easily operated method to test early missed abortion chorionic villi chromosomal abnormalities.
